Quantification of endogenous aromatic cytokinins in Pinus radiata embryonal masses after application of heat stress duri
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SHORT COMMUNICATION
Quantification of endogenous aromatic cytokinins in Pinus radiata embryonal masses after application of heat stress during initiation of somatic embryogenesis A. Castander‑Olarieta1 · C. Pereira1,2 · I. A. Montalbán1 · A. Pěnčík3 · I. Petřík3 · I. Pavlović3 · O. Novák3 · M. Strnad3 · P. Moncaleán1 Received: 11 June 2020 / Accepted: 6 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key Message Short-term exposure to high temperatures modulates the aromatic cytokinin profile of radiata pine embryonal masses when measured 8 weeks after initiation. Abstract Recent research has demonstrated that cytokinins could be involved in abiotic stress responses. However, little research has been published about the role of endogenous aromatic cytokinins during these processes. Therefore, in this study, different temperature and incubation periods (23 ºC, 8 weeks; 40 ºC, 4 h; 50 ºC, 30 min; 60 ºC, 5 min) were applied during induction of radiata pine somatic embryogenesis and the aromatic cytokinin content of the resulting embryonal masses was measured. Results indicated that temperature during induction had a significant effect on the profile of most of the aromatic cytokinins detected. All tested higher temperatures than control provoked a decline in some free bases, including N6-benzyladenine, ortho-Topolin and para-Topolin. On the other hand, the values of N6-benzyladenine nucleotides, ribosides and irreversible metabolites (9G), as well as para-Topolin ribosides, increased in embryonal masses originating from 40 ºC treatment. These results suggest that despite not being traditionally considered part of the stress response machinery, aromatic cytokinins might be involved in heat-stress responses during early stages of somatic embryogenesis in radiata pine. Keywords Embryogenic tissue · N6-Benzyladenine · Orto-topolin · Para-topolin · Radiata pine · Temperature Abbreviations CK Cytokinins SE Somatic embryogenesis EM Embryonal mass ECL Established cell line
Communicated by Klimaszewska. I. A. Montalbán and P. Moncaleán contributed equally as thesis co-directors. * A. Castander‑Olarieta [email protected] 1
Department of Forestry Science, NEIKER, 01192 Arkaute, Spain
2
Department of Life Sciences, Universidade de Coimbra, 3000‑456 Coimbra, Portugal
3
Laboratory of Growth Regulators, Faculty of Science, Institute of Experimental Botany of the Czech Academy of Sciences, Palacký University, 783 71 Olomouc, Czech Republic
BA N6-Benzyladenine BAR N6-Benzyladenosine BARMP N6-Benzyladenosine-5′monophosphate BA7G N6-Benzyladenine-7-glucoside BA9G N6-Benzyladenine-9-glucoside oT ortho-Topolin oTR ortho-Topolin riboside oT7G ortho-Topolin-7-glucoside oT9G ortho-Topolin-9-glucoside mT meta-Topolin mTR meta-Topolin riboside mT7G meta-Topolin-7-glucoside mT9G meta-Topolin-9-glucoside pT para-Topolin pTR para-Topolin riboside pT7G para-Topolin-7-glucoside pT9G para-Topolin-9-glucoside K Kinetin KR Kinetin riboside K9G Kinetin-9-
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