Transcriptome analysis of low phosphate stress response in the roots of masson pine ( Pinus massoniana ) seedlings
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ORIGINAL ARTICLE
Transcriptome analysis of low phosphate stress response in the roots of masson pine (Pinus massoniana) seedlings Xiaocheng Pan1,2 · Haibo Hu1 Received: 13 December 2019 / Revised: 15 November 2020 / Accepted: 20 November 2020 / Published online: 23 November 2020 © Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2020
Abstract Masson pine (Pinus massoniana) is primarily present within subtropical and tropical areas in China, and a number of these regions have a severe deficiency in inorganic phosphate (Pi). As a macronutrient, phosphorus plays a crucial role in plant development. Although several studies have documented the responses of masson pine to Pi starvation at a global level using RNA-Seq and comparative proteomic analyses, the detailed features in the roots that primarily respond to low Pi stress have not yet been studied. Our study examined the response of masson pine roots to a deficiency in Pi. Approximately 1117 unigenes were shown to respond to Pi deficiency by differential expression when analyzed using RNA-Seq. A total of 819 and 298 of these transcripts were up- and down-regulated, respectively. Several transporters including phosphate transporters (PHT1, PHO88), ABC transporters and metal transporters were identified. The ethylene response factor (ERF) was found to be the most abundant transcription factor. Analyses of these genes, including gene ontology enrichment and the KEGG pathway analysis, indicated that the metabolic processes are the most enriched under abiotic stresses, including Pi deficiency. This study provided abundant transcriptomic information to functionally dissect the response of masson pine roots to Pi deficiency. This will additionally aid to elucidate the biological regulatory mechanisms that the pines use to respond to low Pi stress. Keywords Masson pine · Low phosphate · Transcriptome analysis · Gene ontology enrichment Abbreviations Pi Inorganic phosphate ERF Ethylene response factor PHR1 Phosphate starvation response1 SPX SYG1/PHO81/XPR1 PAPs Purple acid phosphatases DEGs Differentially expressed genes FDR False discovery rate ABC ATP-binding cassette
Communicated by Enrico Schleiff. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11738-020-03167-0) contains supplementary material, which is available to authorized users. * Haibo Hu [email protected] 1
Nanjing Forestry University, Nanjing 210000, Jiangsu, China
Zhejiang Agriculture and Forestry University, Linan 311300, Zhejiang, China
2
ROS Reactive oxygen species AP2 Apetala2
Background The masson pine (Pinus massoniana) is a native gymnosperm from the southern portions of China. This tree has broad economic value for its pulp, resin, and timber production. The distribution area of this tree species is primarily in acid yellow soils that critically lack inorganic phosphate (Pi) (Zhang et al. 2010). Phosphorus, in the form of Pi, is an essential macronutrient in plants, which play a vital role in
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