Rapid and Complete Biodegradation of Acrylic Acid by a Novel Strain Rhodococcus ruber JJ-3: Kinetics, Carbon Balance, an
- PDF / 863,284 Bytes
- 10 Pages / 609 x 794 pts Page_size
- 114 Downloads / 197 Views
pISSN 1226-8372 eISSN 1976-3816
RESEARCH PAPER
Rapid and Complete Biodegradation of Acrylic Acid by a Novel Strain Rhodococcus ruber JJ-3: Kinetics, Carbon Balance, and Degradation Pathways Jinjia He, Yi Chen, Luyao Dai, Jiachao Yao, Yu Mei, Dzmitry Hrynsphan, Savitskaya Tatsiana, and Jun Chen
Received: 4 December 2019 / Revised: 21 February 2020 / Accepted: 9 March 2020 © The Korean Society for Biotechnology and Bioengineering and Springer 2020
Abstract Acrylic acid is used in various industrial applications but inflicts harm to human health and causes environmental pollution. A new bacterium, identified as Rhodococcus ruber JJ-3, was isolated, which can degrade high concentrations of acrylic acid rapidly and completely. Experimental results showed that the strain can achieve complete degradation of 1000 mg·L−1 acrylic acid in 11 h under the following conditions: pH 7, temperature 35°C, and inoculation quantity 15%. A high concentration of acrylic acid (2000 mg·L−1) can be completely removed in 28 h. According to the Monod model, the maximum specific degradation rate (vmax) and half saturation rate constant (KS) of the strain were 0.85 h−1 and 101.83 mg·L−1, respectively. The results of carbon balance revealed that 54.6% carbon was assimilated by R. ruber JJ-3 as biomass, and 43.0% carbon was mineralized into CO2. Furthermore, glycerol and lactic acid were measured as intermediates, and the possible degradation pathway was proposed during the biodegradation of acrylic acid. These results suggested that R. ruber JJ-3 completely degrades acrylic acid and might have a potential environmental implication in the purification of acrylic acid-contaminated
Jinjia He, Yi Chen, Luyao Dai College of Environment, Zhejiang University of Technology, Hangzhou 310032, China Jiachao Yao, Yu Mei, Jun Chen* College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 30021, China Tel: +86-571-88285209; Fax: +86-571-88285209 E-mail: [email protected] Dzmitry Hrynsphan, Savitskaya Tatsiana Research Institute of Physical and Chemical Problems, Blearusian State University, Minsk 220030, Belarus
environments. Keywords: acrylic acid, biodegradation, Monod model, carbon balance, degradation pathways
1. Introduction Acrylic acid is an important chemical raw material which has been used in the synthesis of various polymers, such as plastic, synthetic resin, adhesive, leather, textile, and printing [1,2]. Acrylic acid is toxic and is thus listed in the preliminary list of carcinogens published by the World Health Organization and the International Agency for Research on Cancer [3]. In China, financial reports indicate that the annual production of acrylic acid is over 1.2 million tons [4], and approximately 1.4 million tons of acrylic acid wastewater is produced, which has a high pollutant concentration, is highly toxic and difficult to degrade, and causes considerable harm to the environment [5]. In recent decades, various techniques have been developed for acrylic acid wastewater treatment, such as incinera
Data Loading...
