Rapid Detection of Microbial Mass Spectra VITEK-MS for Campylobacter jejuni and Listeria monocytogenes
- PDF / 1,150,644 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 58 Downloads / 282 Views
Rapid Detection of Microbial Mass Spectra VITEK-MS for Campylobacter jejuni and Listeria monocytogenes Tu Bowen 1 & Xue Yingang 2 & Jingchang Xue 1
&
Du Qiang 1 & Li Junhong 1 & Tang Hongbing 1 & Han Xiaodong 3
Received: 13 September 2018 / Accepted: 25 October 2019 # Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract A matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) method were established to detect Campylobacter jejuni and Listeria monocytogenes from a chicken farm and samples from commercial live chickens. Suspected isolates were pretreated and crystallized by matrix solutions consisting of ethanol, acetonitrile, and water with different proportions. The effect of matrix composition was evaluated by examining the differences in characteristic peak values and relative abundances. Significantly different matrix solutions were needed for the detection of C. jejuni and L. monocytogenes: the former did not require lysis by formic acid and was sensitive to trifluoroacetic acid (TFA), but the latter was just the opposite. Control of the water content in matrix solution, TFA concentration, and matrix concentration will enhance the number and relative abundance of characteristic peaks and improve the detection rate. An optimized pretreatment enables the rapid identification of foodborne pathogens, which will make it possible to improve the timeliness of disease prevention and foodborne risk monitoring. Keywords VITEK-MS microbe identification system . Campylobacter jejuni . Listeria monocytogenes . Matrix solution composition . Food risk monitoring
Introduction With governments around the world devoting ever-increasing attention to food safety, work on identification of foodborne pathogens has significantly increased. More and more foodborne pathogenic bacteria have been identified rapidly and efficiently. Indeed, the technology of rapid detection of pathogens has been a highlight of recent food safety research. Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) is a relatively new addition to the technology of rapid microbial detection. More and more Tu Bowen and Xue Yingang contributed equally to this work. * Jingchang Xue [email protected] 1
Changzhou Center for Disease Control and Prevention, Changzhou, China
2
Key Laboratory of Environmental Protection of Water Environment Biological 10 Monitoring of Jiangsu Province, Changzhou Environmental Monitoring Center, Changzhou, China
3
School of Medical, Nanjing University, Nanjing, China
biomarkers have made the identifications of microbes by mass spectrometry ever more accurate and reliable (Van Ert et al. 2004; Briese et al. 2005). Ribosomal proteins have been the predominant molecular markers of MALDI-TOF MS (Suh et al. 2005; Christensen et al. 2012). In addition, enzyme, outer membrane proteins, and virulence-associated proteins provide characteristic peak marks at the species level (Xiao et al. 2012). The recognition accuracy rate at the species
Data Loading...
