Campylobacter jejuni Methods and Protocols

​ Campylobacter jejuni (C. jejuni) is often regarded as the one of the most common causes of bacterial gastroenteritis worldwide. The goal of this volume is to highlight key protocols for working with C. jejuni. In particular, chapters aim to highlig

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James Butcher Alain Stintzi Editors

Campylobacter jejuni Methods and Protocols

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Campylobacter jejuni Methods and Protocols

Edited by

James Butcher and Alain Stintzi University of Ottawa, Ottawa, Canada

Editors James Butcher University of Ottawa Ottawa, Canada

Alain Stintzi University of Ottawa Ottawa, Canada

Videos to this book can be accessed http://www. link.springer.com/videos/[978-1-4939-6536-6] ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6534-2 ISBN 978-1-4939-6536-6 (eBook) DOI 10.1007/978-1-4939-6536-6 Library of Congress Control Number: 2016956255 © Springer Science+Business Media New York 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

Preface Campylobacter jejuni is one of the most common causes of bacterial gastroenteritis, threatening human health worldwide. C. jejuni infection results in Campylobacteriosis: an acute diarrhoeal disease which ranges from a day of mild watery or bloody diarrhea to severe abdominal pain that can linger for several weeks. C. jejuni is also involved in the development of Guillain–Barré syndrome, a debilitating autoimmune disease that causes muscle weakness/paralysis. C. jejuni colonizes poultry as a commensal organism, and poultry farms must implement extremely stringent biosecurity protocols to prevent flocks from Campylobacter infection. C. jejuni can also colonize bovine and porcine hosts who then shed C. jejuni into waterways and farm fields. C. jejuni has been identified in river streams contaminated with livestock run-off, on ho