Selective Enrichment of Clostridium Spp. by Nutrition Control from Sihe Coal Geological Microbial Communities
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Selective Enrichment of Clostridium Spp. by Nutrition Control from Sihe Coal Geological Microbial Communities Dong Xiao 1 & Xuefang Yuan 2 & Meng Wang 3 & Hailun He 3 & Martial Le Prince Essengue Samboukel 1,4 & Yidong Zhang 1 & Enyuan Wang 1 Received: 21 April 2020 / Accepted: 22 June 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
In the coal biogasification, butyric acid is an important intermediate product. The enrichment of butyric acid-producing bacteria in coal geological methanogens is critical to confirm this assertion. Therefore, to study a method for enrichment of butyric acidproducing bacteria and to explore characteristic factors for evaluating the enrichment effect would be the basis for further strain isolation and metabolomics research. In this study, the nutrition control method was used for the butyric acid-producing bacteria enrichment from concentrated bacteria solution in Sihe coal seam. The characteristic factors’ changes in gas production, gas composition, butyric acid concentration, and pH were observed and analyzed in the experiment. High-throughput sequencing was used as a verification method to validate the medium and genera enrichment effect that can be used for the butyric acid-producing bacteria. Through experimental research and analysis, it was identified that the glucose-sucrose-maltose medium was the beneficial medium to the enrichment of butyric acid-producing bacteria, and the high-throughput sequencing determined that the enriched genera were Clostridium spp. Glucose-sucrose-maltose medium experimental data confirmed that the decrease of CO2 and H2 daily yield, the increase of butyric acid concentration, and the decrease of pH value had a significant positive correlation with the enrichment of Clostridium spp. Keywords Butyric acid . Clostridium spp. . Enrichment culture . Biodiversity conservation . Coal geology
* Dong Xiao [email protected] * Hailun He [email protected] Extended author information available on the last page of the article
Applied Biochemistry and Biotechnology
Introduction Coal biogasification is a process to degrade complex carbon structure into CH4 and CO2 with the collaboration of a variety of microbes in the coal bed. The biogasification technology of coal is of great significance for improving the production capacity of coalbed methane, reducing the environmental pollution of coal combustion, and accelerating the development of clean energy [1–3]. These microbes include zymophyte, hydrolytic bacteria, acid-producing bacteria, and methanogen [4, 5]. Most research on coal geological microbial communities focused on the degradation mechanism of coal and the structure of biome. And researches have been recognized that acetic acid is an intermediate product of coal to form methane [5]. Seldom research related to butyric acid and butyric acid-producing bacteria [2, 6–9]. Our study found that butyric acid was an important intermediate in coal biogasification. Butyric acidproducing bacteria, thereby, is an intermediate bact
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