Simultaneous Multiplexed Detection of Protein and Metal Ions by a Colorimetric Microfluidic Paper-based Analytical Devic
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Original Article
Simultaneous Multiplexed Detection of Protein and Metal Ions by a Colorimetric Microfluidic Paper-based Analytical Device Xiaolu Xiong1,2, Junlin Zhang1, Zhou Wang1, Chenchen Liu1, Wende Xiao1,2, Junfeng Han 1,2,* & Qingfan Shi 1,* Received: 16 May, 2020 / Accepted: 14 September, 2020 / Published online: 27 October, 2020 ⒸThe Korean BioChip Society and Springer 2020
Abstract In order to improve the efficiency of disease diagnosis and environmental monitoring, it is desirable to detect the concentration of proteins and metal ions simultaneously, since the current popular diagnostic platform can only detect proteins or metal ions independently. In this work, we developed a colorimetric microfluidic paper-based analytical device (µPAD) for simultaneous determination of protein (bovine serum albumin, BSA) and metal ions [Fe(III) and Ni(II)]. The µPAD consisted of one central zone, ten reaction zones and ten detection zones in one device, in which reaction solutions were effectively optimized for different types of chromogenic reactions. Fe(III), Ni(II) and BSA can be easily identified by the colored products, and their concentrations are in good accordance with color depth based on the established standard curves. The detection limits are 0.1 mM for Fe(III), 0.5 mM for Ni(II) and 1µM for BSA, respectively. Best of all, we demonstrated the efficiency of the µPAD with accurate detection of Fe(III), Ni (II) and BSA from river water samples within 15 minutes. The µPAD detection is efficient, instrument-free, and easy-to-use, holding great potential for simultaneous detection of cross type analytes in numerous diagnostic fields.
1 Key Laboratory of Advanced Optoelectronic Quantum Architecture and Measurement, Ministry of Education, School of Physics, Beijing Institute of Technology, Beijing, 100081, China 2 Micronano Centre, Beijing Key Lab of Nanophotonics & Ultrafine Optoelectronic Systems, Beijing Institute of Technology, Beijing, 100081, China *Correspondence and requests for materials should be addressed to Junfeng Han ( [email protected]) and Qingfan Shi ( [email protected])
Keywords: Microfluidic paper-based analytical device, Colorimetric detection, Simultaneous, Fe(III), Ni(II), BSA
Introduction Proteins and metal ions are vital in life processes1-2. However, excessive intake of these essential biomolecules can lead to various diseases. For example, excess proteins can hinder functions of liver and kidney,3 the accumulation of iron increases the risk of anemia in the human body4, and a high level of nickel causes a variety of cancers5. Therefore, efficient monitoring proteins and metal ions is urgently needed for human health.6 Many methods have been used to detect the proteins and metal ions in drinking water, food and biological fluids7-11. For instance, the enzyme-linked immunosorbent assay (ELISA) can determine proteins sensitively12, inductively coupled plasma atomic emission spectrometry (ICP-AES) detects multiple metal ions at very low detection limits13-14, the liquid crystal-based c
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