Specific root respiration of three plant species as influenced by storage time and conditions
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METHODS PAPER
Specific root respiration of three plant species as influenced by storage time and conditions Zana A. Lak & Hans Sandén & Mathias Mayer & Boris Rewald
Received: 27 March 2020 / Accepted: 22 June 2020 # The Author(s) 2020
Abstract Aims Specific root respiration (RRS) is a key root trait, determining i.e. nutrient foraging and uptake efficiencies. However, a considerable uncertainty exists regarding the effects of storage time and conditions on RRS measurements. Methods Fine root CO2 efflux rates of three plant types (tree seedling Carpinus betulus, legume Pisum sativum, grass Lolium perenne) were measured as depending on storage time (30–1440 min post-rinsing) and conditions (i.e. attached to plant, warm and cold water storage, and storage under dry conditions). Results Short-term storage conditions (30 min) had a significant effect on measured RRS rates, in specific, RRS rates of all three species were significantly lower under dry storage. Irrespective of plant species or temperature, storage of excised roots in water did not affect RRS for 300 min,. RRS measurements remained stable for 1 day if roots were stored cold. Responsible Editor: Amandine Erktan. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11104-020-04619-9) contains supplementary material, which is available to authorized users. Z. A. Lak : H. Sandén : M. Mayer : B. Rewald (*) Institute of Forest Ecology, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences Vienna, Peter-Jordan-Strasse 82, 1190 Vienna, Austria e-mail: [email protected] Z. A. Lak Agriculture College, Forestry Department, Salahaddin University, Kurdistan region, Erbil, Iraq
Conclusions Our results have important implications on m ea s urem e nt rout i ne s of RR S —a gene rally understudied root trait. Henceforth it seems reasonable to collect roots in the field and transport them, hydrated but even uncooled, to the laboratory for subsequent measurements for at least 300 min post-rinsing. Keywords Excised plant roots . Root respiration . Root traits . Sample storage . Specific root respiration
Introduction Root respiration (RR) is a major component of the terrestrial carbon cycle, contributing 10–90% to total soil respiration (Bond-Lamberty et al. 2004; Hanson et al. 2000). In certain cases, RR can account for up to 75% of the assimilated carbon allocated to roots (Majdi et al. 2007), and extensive carbon costs of RR have been directly related to growth above ground (e.g. Rewald et al. 2016). Specific root respiration (RRS) is defined as the amount of CO2 released or O2 absorbed per unit root during a given time; RRS originates from three physiological processes: ion mobilization and uptake, growth and defence, and cell maintenance (Van der Werf et al. 1994). Although respiration of root-associated microbes such as mycorrhizal fungi can contribute to measured RRS rates (Bulgarelli et al. 2012; Nielsen et al. 1998), the specific carbon or O2 costs per unit root indicate carbon- o
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