Testing the Concept of the Interatomic Status of the NFE2L2/AP1 Pathway as a Systemic Biomarker for Examination Stress
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ORIGINAL RESEARCH ARTICLE
Testing the Concept of the Interatomic Status of the NFE2L2/AP1 Pathway as a Systemic Biomarker for Examination Stress Petr V. Zolotukhin • Anastasiya D. Dovzhik • Uliya A. Lebedeva • Oxana N. Kuzminova • Elena V. Mashkina • Anzhela A. Aleksandrova Tatyana P. Shkurat
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Ó Springer International Publishing Switzerland 2014
Abstract Background and Objectives Oxidative status-based interactomic profiling is a promising approach for fundamental integrative cell biology, diagnostics, and therapy. However, this approach has been neither utilized as a method nor tested as a tool. Thus, we aimed (1) to develop an oxidative status pathway state assessment-based analytical procedure relying on NFE2L2/AP1 pathway evaluation, and (2) to preliminarily assess its responsiveness, performance and diagnostic properties when applied to deciphering stress conditions of the academic examination period and academic term. These conditions were chosen as those representing a common model of mild, everydaylife stressors causing shifts in oxidative status. Methods To meet the aim of the study, we performed a repetitive-measurements study collating gene expression of NFE2L2/AP1 pathway targets and controllers under the two stress conditions using semi-quantitative reverse transcription-polymerase chain reaction. Results Surprisingly, even with some sensitivity limitations of the methods employed, a pathway state analysis
P. V. Zolotukhin (&) A. D. Dovzhik U. A. Lebedeva O. N. Kuzminova A. A. Aleksandrova Laboratory of Biomedicine, Research Institute of Biology, Southern Federal University, Stachki av., 194/1, 344090 Rostov-on-Don, Russia e-mail: [email protected] E. V. Mashkina Laboratory of Human Genetics, Research Institute of Biology, Southern Federal University, Stachki av., 194/1, 344090 Rostov-on-Don, Russia T. P. Shkurat Research Institute of Biology, Southern Federal University, Stachki av., 194/1, 344090 Rostov-on-Don, Russia
approach based on a multiple target-to-controller ratio calculation was highly responsive and yielded very high receiver operating characteristics in deciphering the model stress conditions. Conclusion Although further testing of the approach is required, the interactomic pathway activation assaying concept was preliminarily experimentally proven to be a highly promising clinical diagnostic tool that may easily be adapted for current tasks.
1 Introduction Oxidative status of human cells and their surrounding, defined as the qualitative and quantitative superposition of pro- and antioxidants, is considerably involved in a broad variety of cellular-level processes [1, 2]. Reactive oxygen species (ROS) production substantially represents metabolic activity of cellular compartments and, simultaneously, performance of respective defensive mechanisms, and this fact might have predisposed ROS signaling functions. Oxidative status systems include intracellular/environmental-condition sensors capable of triggering adaptive pathways and major cellular signaling pathways [3].
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