The determination of a novel inducible WY172 promoter derived from Oidium heveae HO-73
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ORIGINAL ARTICLE
The determination of a novel inducible WY172 promoter derived from Oidium heveae HO‑73 Jinyao Yin1,2 · Yi Wang1,2 · Li Zhu1,2 · Chen Wang1,2 · Jiyuan Wang1,2 · Wenbo Liu1,2 · Chunhua Lin1,2 · Xiao Li1,2 · Weiguo Miao1,2 Received: 11 February 2020 / Accepted: 25 August 2020 / Published online: 22 September 2020 © Springer Nature B.V. 2020
Abstract In plant genetic engineering, to ensure the efficiently expression of foreign genes in recipient plant cells require high-quality promoters. In this study, a novel promoter, WY172, derived from Oidium heveae B.A. Steinm, was isolated and functionally characterized. GUS staining and GUS activity tests indicated that WY172 could effectively drive GUS expression in both monocotyledonous rice and dicotyledonous tobacco. The micro-hypersensitive response revealed that transgenic tobacco harbouring WY172 and HpaXm had more blue necrotic cells than that of positive control of CaMV35S-HpaXm transgenic tobacco. As well, TMV inoculation tests showed fewer lesions on the leaves of WY172-HpaXm transgenic tobacco than those of the positive control, indicating that WY172 could drive the expression of exogenous functional genes HpaXm. Furthermore, the expression level of the HpaXm gene in WY172-HpaXm transgenic tobacco after TMV inoculation was 7.1 times that of uninoculated tobacco. And WY172 also had a relative stronger ability to drive expression of HpaXm after TMV inoculation compared with non-inoculated plants, indicating that WY172 might be a pathogen-inducible promoter. Bioinformatics analysis indicated that WY172 is rich in pathogen-induced cis-acting elements such as auxin response element and light response elements, and induction experiments demonstrated that WY172 drives GUS expression after exogenous application of IAA, which both supported that WY172 might be an inducible promoter. In conclusion, WY172 promoter is an IAA- and pathogen-inducible promoter, which might has high potential for use in a wide range of hosts. Key message By blasting and predicting the Oidium heveae B.A. Steinm genome, we obtained and preliminarily demonstrated that the WY172 promoter that may be induced by IAA and TMV. WY172 can play a role in rice and tobacco, and can drive efficient expression of HpaXm in transgenic tobacco and produce TMV resistance and micro-hypersensitive response. Keywords WY172 · Inducible promoter · GUS · ATMT · Micro-HR · TMV · IAA
Communicated by Degao Liu. Jinyao Yin and Yi Wang contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11240-020-01923-1) contains supplementary material, which is available to authorized users. * Weiguo Miao [email protected] 1
Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Hainan University), Ministry of Education, Haikou 570228, China
College of Plant Protection, Hainan University, Haikou 570228, China
2
Abbreviations ATMT Agrobacterium tumefaciens-mediated transformation Micro-HR Micro-hype
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