The landscape of host genetic factors involved in immune response to common viral infections
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RESEARCH
Open Access
The landscape of host genetic factors involved in immune response to common viral infections Linda Kachuri1†, Stephen S. Francis1,2,3,4*†, Maike L. Morrison5,6,7, George A. Wendt2, Yohan Bossé8, Taylor B. Cavazos9, Sara R. Rashkin1,10, Elad Ziv3,11,12 and John S. Witte1,3,5,12,13*
Abstract Background: Humans and viruses have co-evolved for millennia resulting in a complex host genetic architecture. Understanding the genetic mechanisms of immune response to viral infection provides insight into disease etiology and therapeutic opportunities. Methods: We conducted a comprehensive study including genome-wide and transcriptome-wide association analyses to identify genetic loci associated with immunoglobulin G antibody response to 28 antigens for 16 viruses using serological data from 7924 European ancestry participants in the UK Biobank cohort. Results: Signals in human leukocyte antigen (HLA) class II region dominated the landscape of viral antibody response, with 40 independent loci and 14 independent classical alleles, 7 of which exhibited pleiotropic effects across viral families. We identified specific amino acid (AA) residues that are associated with seroreactivity, the strongest associations presented in a range of AA positions within DRβ1 at positions 11, 13, 71, and 74 for EpsteinBarr virus (EBV), Varicella zoster virus (VZV), human herpesvirus 7, (HHV7), and Merkel cell polyomavirus (MCV). Genome-wide association analyses discovered 7 novel genetic loci outside the HLA associated with viral antibody response (P < 5.0 × 10−8), including FUT2 (19q13.33) for human polyomavirus BK (BKV), STING1 (5q31.2) for MCV, and CXCR5 (11q23.3) and TBKBP1 (17q21.32) for HHV7. Transcriptome-wide association analyses identified 114 genes associated with response to viral infection, 12 outside of the HLA region, including ECSCR: P = 5.0 × 10−15 (MCV), NTN5: P = 1.1 × 10−9 (BKV), and P2RY13: P = 1.1 × 10−8 EBV nuclear antigen. We also demonstrated pleiotropy between viral response genes and complex diseases, from autoimmune disorders to cancer to neurodegenerative and psychiatric conditions. Conclusions: Our study confirms the importance of the HLA region in host response to viral infection and elucidates novel genetic determinants beyond the HLA that contribute to host-virus interaction. Keywords: Infection, Virus, Serology, Antigen, Antibody, Immunoglobulin G, Immune response, Human leukocyte antigen (HLA), Polyomavirus, Genome-wide association study (GWAS), Transcriptome-wide association study (TWAS)
* Correspondence: [email protected]; [email protected] † Linda Kachuri and Stephen S. Francis contributed equally to this work. 1 Department of Epidemiology and Biostatistics, University of California San Francisco, San Francisco, CA, USA Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in
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