Toxoplasma invasion delayed by Tg ERK7 eradication
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PROTOZOOLOGY - ORIGINAL PAPER
Toxoplasma invasion delayed by TgERK7 eradication Zhong-Yuan Li 1
&
Xue Liang 1 & Hai-Ting Guo 1 & Jie Tan 1 & Xing-Quan Zhu 2 & Quan Liu 3,4
Received: 22 July 2020 / Accepted: 7 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Toxoplasma gondii causes serious clinical toxoplasmosis in humans mostly due to its asexual life cycles, which can be artificially divided into five tightly coterminous stages. Any radical or delay for the stage will result in tremendous changes immediately behind. We previously demonstrated that TgERK7 is associated with the intracellular proliferation of T. gondii, but during the process, other stages before were not meanwhile determined. To further clarify the function of ERK7 gene in T. gondii, the complemental strain of ΔTgERK7 tachyzoites created previously was engineered via electric transfection with the recombinant pUC/Tgerk7 plasmid, named pUC/TgERK7 strain in this study, and was used together with ΔTgERK7 and wild-type GT1 strains to retrospect the phenotypic changes including invasion and attachment. The results showed that TgERK7 protein can be re-expressed in the ΔTgERK7 tachyzoites and eradication of this protein leads to significantly lower invasion of T. gondii at 1 h and 2 h post-infection (P < 0.05), which is the key factor causing the following slow intracellular proliferation, in comparison with wild-type GT1 and pUC/TgERK7 parasites; noteworthily, at other early time points including 15 min for attachment assay was no statistical difference (P > 0.05). The data suggested that ERK7 protein in T. gondii is an important virulence factor that participates in the invasion of this parasite. Keywords Toxoplasma gondii . TgERK7 . Eradication . Complemental expression . Invasion
Introduction Toxoplasma gondii with the extremely widespread distribution is an important opportunistic protozoan parasite that possesses two types of life cycles, the sexual in feline and the Handling Editor: Una Ryan * Zhong-Yuan Li [email protected]; [email protected] 1
Guangxi Key Laboratory of Brain and Cognitive Neuroscience, Guilin Medical University, Guilin 541199, Guangxi Zhuang Autonomous Region, People’s Republic of China
2
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu Province, People’s Republic of China
3
Military Veterinary Institute, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Academy of Military Medical Sciences, Changchun 130122, Jilin Province, People’s Republic of China
4
School of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong Province, People’s Republic of China
asexual in its intermediate hosts such as animals and human beings (Hehl et al. 2015; Pittman and Knoll 2015). Though normally relatively benign for immunocompetent individuals, T. gondii can also lead to serious clin
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