Unpredictable recombination of PB transposon in Silkworm: a potential risk
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ORIGINAL ARTICLE
Unpredictable recombination of PB transposon in Silkworm: a potential risk Xuehua Jia1,2 · Xiaoyu Pang1,2 · Yajie Yuan1 · Qiang Gao1 · Ming Lu2 · Guangxian Zhang2 · FangYing Dai1,2,3 · Tianfu Zhao1,2 Received: 24 July 2020 / Accepted: 27 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The piggyBac (PB) transposon is the most widely used vector for generating transgenic silkworms. The stability of the PB transposon in the receptor is a serious concern that requires attention because of biosafety concerns. In this study, we found that the transgene silkworm developed loss of reporter gene traits. To further investigate the regularity, we traced the genes and traits of this silkworm. After successful alteration of the silkworm genome with the MASP1 gene (named red-eyed silkworm; RES), silkworm individuals with lost reporter genes were found after long-term transgenerational breeding and were designated as the white-eyed silkworm (WES). PCR amplification indicated that exogenous genes had been lost in the WES. Testing was conducted on the PB transposons, and the left arm (L arm) did not exist; however, the right arm (R arm) was preserved. Amino acid analysis showed that the amino acid content of the WES changed versus the common silkworm and RES. These results indicate that the migration of PB transposons in Bombyx mori does occur and is unpredictable. This is because the silkworm genome contains multiple PB-like sequences that might influence the genetic stability of transgenic lines. When using PB transposons as a transgene vector, it is necessary to fully evaluate and take necessary measures to prevent its re-migration in the recipient organism. Further experiments are needed if we want to clarify the regularity of the retransposition phenomenon and the direct and clear association with similar sequences of transposons. Keywords piggyBac · Silkworm · Bombyx mori · Transposon stability · Transgenic
Background Bioengineering technology is widely used in the production of new strains, biomass materials, and bioreactors. Some new lines have been created using transgenic methods of insects, plants, fish, mammals, and other species (Handler 2002). There are concerns about the biosafety of genetically Communicated by Stefan Hohmann. * Tianfu Zhao [email protected] 1
State Key Laboratory of Silkworm Genome Biology, College of Textile and Garment, Southwest University, No. 2 Tiansheng Street, Beibei, Chongqing 400715, China
2
Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile, Chongqing 400715, China
3
Key Laboratory of Sericultural Biology and Genetic Breeding, College of Biotechnology, Southwest University, Chongqing 400715, China
modified organisms (GMOs) that involve several aspects. First, GMOs (genetically modified organisms) bring the risk of ecological balance. Second, the risks of unplanned changes in exogenous genes in the receptor are unpredictable. The cargo frequently used for gene delivery is largely divided into
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