Use of rosiglitazone before and after vascular injury in hypercholesterolemic rabbits: Assessment of neointimal formatio
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BioMed Central
Open Access
Original basic research
Use of rosiglitazone before and after vascular injury in hypercholesterolemic rabbits: Assessment of neointimal formation Alexandre Alessi, Olímpio Ribeiro França Neto, Paulo Roberto Slud Brofman, Camila Prim, Lucia Noronha, Ruy Fernando Kuenzer Caetano Silva, Liz Andréa Villela Baroncini* and Dalton Bertolim Précoma Address: Center of Health and Biological Sciences, Pontifical Catholic University of Paraná, Brazil Email: Alexandre Alessi - [email protected]; Olímpio Ribeiro França Neto - [email protected]; Paulo Roberto Slud Brofman - [email protected]; Camila Prim - [email protected]; Lucia Noronha - [email protected]; Ruy Fernando Kuenzer Caetano Silva - [email protected]; Liz Andréa Villela Baroncini* - [email protected]; Dalton Bertolim Précoma - [email protected] * Corresponding author
Published: 27 August 2008 Thrombosis Journal 2008, 6:12
doi:10.1186/1477-9560-6-12
Received: 3 June 2008 Accepted: 27 August 2008
This article is available from: http://www.thrombosisjournal.com/content/6/1/12 © 2008 Alessi et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Objectives: To analyse the effects of rosiglitazone administered at different times on neointimal formation in hypercholesterolemic rabbits following vascular injury. Methods: Thirty-nine rabbits on a hypercholesterolemic diet were included. The animals underwent balloon catheter injury to the right iliac artery on day 14. They were divided into three groups as follows: control group, 13 rabbits without rosiglitazone; group I, 13 rabbits treated with rosiglitazone (3 mg/Kg body weight/day) for 28 days after the vascular injury; and group II, 13 rabbits treated with rosiglitazone (3 mg/Kg body weight/day) during all the experiment (42 days). Histological analysis was done by an experienced pathologist who was unaware of the rosiglitazone treatment. Histomorphometric parameters were performed by calculation of the luminal and intimal layer area, and intima/media layer area ratio (the area of the intimal layer divided by the area of the medial layer). Results: Intimal area was significantly lower in group II vs. CG (p = 0.024) and group I (p = 0.006). Luminal layer area was higher in group II vs. CG (p < 0.0001) and group I (p < 0.0001). Intima/media layer area ratio was equal between CG and group I. Intima/media layer ratio area was significantly lower in group II vs. control group (p < 0.021) and group I (p < 0.003). There was a significant reduction of 65% and 71% in intima/media layer area ratio in group II vs. control group and group I, respectively. Conclusion: Pretreatment with rosiglitazone in hypercholesterolemic rabbits submitted to vascular injury significantly reduces neointimal formation.
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