Utilizing quantitative immunohistochemistry for relationship analysis of tumor microenvironment of head and neck cancer
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POSTER PRESENTATION
Open Access
Utilizing quantitative immunohistochemistry for relationship analysis of tumor microenvironment of head and neck cancer patients Zipei Feng1*, Tarsem Moudgil1, Allen Cheng1, Christopher Paustian1, Rieneke van de Ven1, Christopher Dubay1, Hong-Ming Hu1, Tuan Bui2, Tyler Hulett1, Traci Hilton3, Carlo Bifulco1, Richard B Bell2, Bernard A Fox1,4 From Society for Immunotherapy of Cancer 29th Annual Meeting National Harbor, MD, USA. 6-9 November 2014 Background Analysis of tumor-infiltrating immune cells using quantitative immunohistochemistry (IHC) has proved to be a powerful prognostic biomarker in colon cancer [1,2]. Similar observations have been made in patients with oral, head and neck squamous cell carcinoma (OHNSCC), where CD8 infiltration is associated with prolonged survival [3]. Recently, advancements are made in multiplex imaging and relationship analysis to better delineate suppressive mechanisms within the tumor microenvironment, which may direct immune interventions that augment tumor-specific immune response. Purpose The purpose of this investigation was to apply multiplex immunohistochemistry and objective assessment techniques to identify biomarkers that correlate with HPV status, T cell infiltrate, and patient survival. Relationships analysis between immune markers and tumor cells will also be performed to examine the dynamic interactions that occur within the tumor microenvironment. Methods 92 subjects with biopsy-proven OHNSCC from different sub-sites underwent surgery with curative intent and were enrolled into this prospective, IRB approved protocol. Formalin-fixed-paraffin-embedded (FFPE) samples of patients’ primary tumor or metastatic lymph nodes are obtained and stained for markers including CD4, CD8, CD137, CD163, interferon-gamma, arginase I, PD-L1, and class I, using the PerkinElmer Opal system. Images are scanned 1 Earle A. Chiles Research Institute, Providence Cancer Center; Department of Cancer Biology, Oregon Health & Science University, Portland, OR, USA Full list of author information is available at the end of the article
Figure 1 Arg1: HPV correlation.
and analyzed using PerkinElmer Vectra system. Single stains are being done simultaneously using Ventana Benchmark XT and analyzed using Definiens platform.
Results Preliminary results analyzed from 24 patients showed positive correlation between CD8 immune infiltrate within the tumor and HPV status (P = 0.05). Level of Arg1 within the tumor microenvironment showed a stronger correlation with HPV status (Figure 1), and inversely correlated with CD8 infiltrate (P = 0.03). Interestingly, the number of IFN-g positive CD8 cells has no correlation with PD-L1 status in the subset of the patients that we have analyzed
© 2014 Feng et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the orig
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