QTLs associated with resistance in soybean PI567516C to synthetic nematode population infecting cv. Hartwig
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J. Crop Sci. Biotech. 2010 (September) 13 (3) : 163 ~ 167 DOI No. 10.1007/s12892-010-0060-z RESEARCH ARTICLE
QTLs Associated with Resistance in Soybean PI567516C to Synthetic Nematode Population Infecting cv. Hartwig Prakash R. Arelli1*, Vergel C. Concibido2, Lawrence D. Young3 USDA-ARS Mid South Area, Jackson, TN 38301 USA Monsanto Company, St. Louis, MO 63167 USA 3 USDA-ARS Mid South Area, Stoneville, MS 38776 USA
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Received: May 27, 2010 / Revised: June 20, 2010 / Accepted: September 10, 2010 Ⓒ Korean Society of Crop Science and Springer 2010
Abstract Worldwide, soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is the most destructive pathogen of soybean [Glycine max (L.) Merr.]. Crop losses are primarily mitigated by the use of resistant cultivars. Nematode populations are variable and have adapted to reproduce on resistant cultivars over time because resistance primarily traces to two soybean accessions, Plant Introduction (PI) 88788 and Peking. Soybean cultivar Hartwig, derived primarily from PI437654, was released for its comprehensive resistance to most SCN populations. A synthetic nematode population (LY1) was recently selected for its reproduction on Hartwig. The LY1 nematode population currently infects known sources of resistance except soybean PI567516C; however, the resistance to LY1 has not been characterized. The objective of this study was to identify quantitative trait loci (QTLs) underlying resistance to the LY1 SCN population in PI567516C, identify diagnostic DNA markers for the LY1 resistance, and confirm their utility for markerassisted selection (MAS). Resistant soybean line PI567516C was crossed to susceptible cultivar Hartwig to generate 105 recombinant inbred lines (F2-derived F5 families). QTLs were mapped using simple sequence repeats (SSRs) covering 20 Linkage Groups (LGs) and three diagnostic markers, Satt592, Satt331, and Sat_274, were identified on LG O. These markers have a combined efficacy of 90% in identifying resistant lines in a second cross that has been generated by crossing a susceptible cultivar 5601T with resistant PI567516C. F2-derived F4 segregating population was used in MAS to identify resistant lines. Key words: bioassay, breeding, cyst nematode, Glycine max, Heterodera glycines, mapping, marker assisted selection, resistance, soybean
Introduction Worldwide, soybean [Glycine max (L.) Merr.], grown for its edible protein and oil, is a very important agronomic crop. Soybean cyst nematode, Heterodera glycines Ichinohe (SCN) reduces yield more than any other soybean pest in the world (Wrather et al. 2001). SCN causes yield reductions by feeding on plant nutrients, retarding root growth, and inhibiting Bradyrhizobium nodulation (Arelli et al. 2009). Resistant cultivars have been an effective means of control. Soybean breeders have been evaluating the soybean germplasm collection for sources of resistance. Most evaluations for nemaPrakash R. Arelli ( ) Email: [email protected] Tel: +1 731-425-4741 Fax: +1 -731-425-4760
The Korean Society of Crop S
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