Quantification of SARS-CoV-2 antigen levels in the blood of patients with COVID-19
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antification of SARS-CoV-2 antigen levels in the blood of patients with COVID-19 1†
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Bin Su , Jiming Yin , Xingguang Lin , Tiantian Zhang , Xiao Yao , Ying Xu , Yao Lu , 5,6 2 7 7,8,9* 1* 1* Wenzhi Wang , Kun Liu , Jie Zhang , Liangzhi Xie , Ronghua Jin & Yingmei Feng 1
Beijing Youan Hospital, Capital Medical University, Beijing 100069, China; 2 Hangzhou Gbio Biotechnology Co., Ltd., Hangzhou 310000, China; 3 Hangzhou Neoline Technology Co., Ltd., Hangzhou 310000, China; 4 Quanterix Corporation, 900 Middlesex Turnpike, Billerica, MA 01821, USA; 5 Guizhou Academy of Testing and Analysis, Guiyang 550014, China; 6 Wildlife Forensic Science Service, Kunming 650203, China; 7 Beijing Key Laboratory of Monoclonal Antibody Research and Development, Sino Biological Inc. Beijing 100176, China; 8 Beijing Engineering Research Center of Protein and Antibody, Sinocelltech Ltd., Beijing 100176, China; 9 Cell Culture Engineering Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China Received August 18, 2020; accepted November 11, 2020; published online November 26, 2020
Citation:
Su, B., Yin, J., Lin, X., Zhang, T., Yao, X., Xu, Y., Lu, Y., Wang, W., Liu, K., Zhang, J., Xie, L., Jin, R., and Feng, Y. (2020). Quantification of SARSCoV-2 antigen levels in the blood of patients with COVID-19. Sci China Life Sci 63, https://doi.org/10.1007/s11427-020-1830-8
Dear Editor, Severe acute respiratory syndrome coronavirus 2 (SARSCoV-2) has caused the global outbreak of coronavirus disease 2019 (COVID-19). By far, more than 35 million people had been infected by SARS-CoV-2, resulting in more than 1 million deaths globally. It is well recognized that SARSCoV-2 preferentially attacks pulmonary epithelial cells, leading to acute respiratory distress syndrome (ARDS). Remarkably, infected patients experience liver (Phipps et al., 2020), kidney (Gabarre et al., 2020) or heart injury (Bavishi et al., 2020), indicating the presence of multiple-organ dysfunction via viremia. By far, viral detection in RNA extracted from nasopharyngeal swabs, saliva, sputum, and bronchoalveolar lavage fluid has remained the standard etiological diagnostic procedure for COVID-19, with the detection rate ranging from 70% to more than 90% (Lippi et al., 2020). Paradoxically, the †Contributed equally to this work *Corresponding authors (Yingmei Feng, email: [email protected]; Ronghua Jin, email: [email protected]; Liangzhi Xie, email: [email protected])
viral RNA detection rate is only 15%–30% in blood samples (Scohy et al., 2020; Mak et al., 2020). Moreover, hampered by the low performance of the current technology, few studies have evaluated SARS-CoV-2 antigen levels in the blood. The single molecular array (SIMOA™) ultrasensitive platform employs enzyme-conjugated microscopic beads for protein capture and enables antigen quantification at femtomolar concentrations (Rissin et al., 2010). In the study, we investigated whether the SIMOA method could determine SARS-CoV-2 antigen levels in patients with COV
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