Radiosynthesis of Gold/Albumin Core/shell Nanoparticles for Biomedical Applications
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Radiosynthesis of Gold/Albumin Core/shell Nanoparticles for Biomedical Applications Constanza Y. Flores1, Estefania Achilli1 and Mariano Grasselli 1* 1
Laboratorio de Materiales Biotecnológicos – Grupo vinculado al IMBICE – CCT La Plata, Departamento de Ciencia y Tecnología, UNQ, Roque Saenz Peña 352, Bernal, Bs. As, Argentina
*Corresponding author ABSTRACT Gold/albumin core/shell nanoparticles (Au/AlbNPs) was prepared by a novel aggregation/crosslinking technique and characterized by several spectroscopic and microscopy methods. Albumin, in presence of gold nanoparticles (AuNPs), is aggregated by the addition of ethanol and further stabilized by radiation-induced crosslinking using a 60Co source. Nanoconstructs are characterized to determine size, morphology and optical characteristics. The Au/AlbNPs were prepared in different ethanol and albumins concentrations. Results showed that it is possible to obtain Au/AlbNPs using ethanol 30 %v/v, albumin in different concentrations and an irradiation dose of 10 kGy. Au/AlbNP plasmon peak shifted to 530 nm, keeping the typical plasmon peak shape. The size of Au/AlbNPs is approximately double respect to the naked AuNPs and they show core/shell type morphology. The main amide peaks of albumin in FTIR spectrum can be found in the spectrum of nanoconstructs.
INTRODUCTION Breast cancer is the tumour with the highest incidence and mortality of women in the world; it is for this reason that many investigations are aimed to therapeutic drug design strategies for diagnosis and treatment. Tremendous advances have been made in the treatment, prevention and early detection of these malignancies; however none of the current therapies are specifically able to cover all the variants of this disease that differ in its histopathology characteristics and genomic and genetic variations [1]. In addition, many of the available drugs are not able to reach the site of metastases [2]. For this reason there is a new approach in the development of therapeutic strategies which allow high degree of specificity and spatial extent of the tumours even after metastasis spread. This approach is addressed by nanotechnology [2]. The use of nanotechnology in medicine, also called nanomedicine, is based on the generation of nanostructures, especially in round shape particles or nanoparticles (NPs). Many characteristics of these nanomedicines will depend on the size and surface properties of the NPs. NPs size of currently used in anti-cancer therapy varies between 10-100 nm. An advantage of the use of NPs in such therapies is that the tumor vasculature has higher permeation for
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macromolecules, in addition to the poorly functionality of lymphatic system in the surround media. NPs accumulate in tumors leading to phenomenon known as ‘Effect of enhanced
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