Rapid Isolation and Multiplexed Detection of Exosome Tumor Markers Via Queued Beads Combined with Quantum Dots in a Micr
- PDF / 1,261,562 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 86 Downloads / 181 Views
ARTICLE
Cite as Nano-Micro Lett. (2019) 11:59 Received: 21 April 2019 Accepted: 10 June 2019 © The Author(s) 2019
https://doi.org/10.1007/s40820-019-0285-x
Rapid Isolation and Multiplexed Detection of Exosome Tumor Markers Via Queued Beads Combined with Quantum Dots in a Microarray Yanan Bai1,2, Yunxing Lu1,2, Kun Wang1,2, Zule Cheng1,2, Youlan Qu1,3, Shihui Qiu1, Lin Zhou1, Zhenhua Wu1, Huiying Liu3 *, Jianlong Zhao1 *, Hongju Mao1,2 * Yanan Bai and Yunxing Lu have contributed equally to this work. * Huiying Liu, [email protected]; Jianlong Zhao, [email protected]; Hongju Mao, [email protected] 1 State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, People’s Republic of China 2
3
Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China School of Stomatology, Dalian Medical University, Dalian 116044, People’s Republic of China
HIGHLIGHTS • A bead-based microarray for exosome isolation and multiplexed tumor marker detection was developed. • The beads are uniformly trapped and queued among the micropillars in the chip, which can avoid optical interference and enable more accurate test results. • The results with different types of lung cancer exosome samples showed distinctive marker expression levels. ABSTRACT Tumor-derived exosomes are actively involved in cancer progression and metastasis and have emerged as a promising marker for
cancer diagnosis in liquid biopsy. Because of their nanoscale size, complex
Exosomes
Microbeads
biogenesis, and methodological limitations related to exosome isolation and detection, advancements in their analysis remain slow. Microfluidic technology offers a better analytic approach compared with conventional methods. Here, we developed a bead-based microarray for exosome isolation and mul-
QD probes
Capture antibody
tiplexed tumor marker detection. Using this method, exosomes are isolated by binding to antibodies on the bead surface, and tumor markers on the exosomes are detected through quantum dot (QD) probes. The beads are then uniformly trapped and queued among micropillars in the chip. This design
Multiplexed Detection of Exosome Tumor Markers via Queued Beads
benefits fluorescence observation by dispersing the signals into every single bead, thereby avoiding optical interference and enabling more accurate test results. We analyzed exosomes in the cell culture supernatant of lung cancer and endothelial cell lines, and different lung cancer markers labeled with three QD probes were used to conduct multiplexed detection of exosome surface protein markers. Lung cancer-derived samples showed much higher (~ sixfold–tenfold) fluorescence intensity than endothelial cell samples, and different types of lung cancer samples showed distinctive marker expression levels. Additionally, using the chip to detect clinical plasma samples from cancer patients showed good diagnos
Data Loading...
