Reproductive Endocrinology A Molecular Approach
Modern reproductive biology encompasses every level of biological study from genomics to ecology, encompassing cell biology, biochemistry, endocrinology and general physiology. All of these disciplines require basic knowledge, both as a tool and as a
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Reproductive Endocrinology A Molecular Approach
Reproductive Endocrinology
Pedro J. Chedrese Editor
Reproductive Endocrinology A Molecular Approach
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Editor Pedro J. Chedrese Department of Biology College of Arts and Science University of Saskatchewan Saskatoon, Saskatchewan Canada [email protected]
ISBN 978-0-387-88185-0 e-ISBN 978-0-387-88186-7 DOI 10.1007/978-0-387-88186-7 Library of Congress Control Number: 2008935613 c Springer Science+Business Media, LLC 2009 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper springer.com
Almost all aspects of life are organized at the molecular level, and if we do not understand molecules, our comprehension of life will be very incomplete Francis Crick
Preface
Molecular biology is a relatively new science that emerged from the advances in biochemistry of the 1940s and 1950s, when the structure of the nucleic acids and proteins were elucidated. With progress in the 1970s in nucleic acid enzymology and the discovery of the restriction enzymes, the tools of molecular biology became widely available and were applied to cell biology to study how genes are regulated. This new knowledge impacted endocrinology and reproductive biology, since it was largely known that the secretion of the internal glands affected phenotypes, and therefore expression of genes. In 1982 the first edition of “Molecular Cloning: A Laboratory Manual,” (Maniatis, Fritch and Sambrook) was published, offering researchers the first organized collection of protocols for molecular techniques. It was the application of these techniques by endocrinologists that gave birth to molecular endocrinology. From then on, the use of these modern tools not only shed light on fundamental questions in endocrinology and reproductive biology, but also has become central to scientific studies in all living matter, impacting disciplines such as human and veterinary medicine and related health sciences, including animal, agriculture and environmental sciences. Thus, modern re
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