Response of extracellular and intracellular alkaline phosphatase in Microcystis aeruginosa to organic phosphorus
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CYANOBACTERIAL BLOOMS AND WATER ECOLOGICAL RESTORATION
Response of extracellular and intracellular alkaline phosphatase in Microcystis aeruginosa to organic phosphorus Tingxi Zhang 1,2,3 & Xiaoran Lu 1 & Rongda Yu 1 & Mengyao Qin 1 & Chao Wei 1 & Sujuan Hong 4 Received: 9 October 2019 / Accepted: 15 June 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Cyanobacterial blooms caused by Microcystis have become a menace to public health and water quality in the global freshwater ecosystem. Alkaline phosphatases (APases) produced by microorganisms play an important role in the mineralization of dissolved organic phosphorus (DOP) into orthophosphate (Pi) to promote cyanobacterial blooms. However, the response of extracellular and intracellular alkaline phosphatase activity (APA) of Microcystis to different DOP sources is poorly understood. In this study, we compared the growth of M. aeruginosa on two DOP substrates (β-glycerolphosphate (β-GP) and lecithin (LEC)) and monitored the changes of P fractions and the extra- and intracellular APA under different P sources and concentrations. M. aeruginosa can utilize both β-GP and LEC to sustain its growth, and the bioavailability of LEC was greater than β-GP. For the β-GP treatment, there was no significant difference in the algal growth at different concentrations (P > 0.05), while the algal growth in the LEC treatment groups was significantly affected by concentrations (P < 0.05). The results showed that intracellular APA of M. aeruginosa could be detected in all DOP treatment groups and generally higher than extracellular APA. In addition, the intracellular APA per cell increased first and then decreased in all DOP treatment groups. Compared with the β-GP treatment, M. aeruginosa in the LEC groups could secret more extracellular APA. Keywords M. aeruginosa . alkaline phosphatase activity . extracellular and intracellular . organic phosphorus . β-glycerol-phosphate . lecithin . mineralization
Introduction Eutrophication in aquatic ecosystems is a major environmental issue, threatening water security and biodiversity. Rongda Yu and Mengyao Qin contributed equally to this work. Responsible Editor: Vitor Manuel Oliveira Vasconcelos * Tingxi Zhang [email protected] 1
School of Environment, Nanjing Normal University, Nanjing 210023, China
2
Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing 210023, China
3
Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Nanjing 210023, China
4
School of Life Sciences, Nanjing Normal University, Nanjing 210046, China
Cyanobacterial blooms mainly caused by Microcystis in freshwater lakes have become more frequent ( Ho et al. 2019; Sato et al. 2017; Schindler et al. 2016; Yang and Kong 2013). Phosphorus (P) is an important requirement for microbial metabolism (Geider and La Roche 2002; Markou et al. 2014) and is a limiting nutrient of lakes primary productivity (Carpenter 2008; McMahon and Read 2013; Sondergaard
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