Reverse Genetics of RNA Viruses Methods and Protocols
This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or
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Daniel R. Perez Editor
Reverse Genetics of RNA Viruses Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Reverse Genetics of RNA Viruses Methods and Protocols
Edited by
Daniel R. Perez Department of Population Health, Poultry Diagnostic and Research Center, College of Veterinary Medicine, University of Georgia, Athens, GA, USA
Editor Daniel R. Perez Department of Population Health, Poultry Diagnostic and Research Center, College of Veterinary Medicine University of Georgia Athens, GA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6962-3 ISBN 978-1-4939-6964-7 (eBook) DOI 10.1007/978-1-4939-6964-7 Library of Congress Control Number: 2017936192 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface The International Committee on Taxonomy of Viruses (ICTV) classifies RNA viruses as those that belong to Group III, Group IV, or Group V of the Baltimore classification system and contain ribonucleic acid (RNA) as genetic material throughout their entire life cycle. Group III includes double-stranded RNA viruses (dsRNAs), whereas Groups IV and V contain single-stranded RNA viruses (ssRNAs) of positive and negative polarity, respectively. Positive sense RNA viruses (+ssRNAs) are those in which the RNA itself is translated by the host cell translation machinery and initiates an infectious cycle de novo. In contrast, negative sense RNA viruses (−s
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