Rhodotorula mucilaginosa Isolated from the Manganese Mine Water in Minas Gerais, Brazil: Potential Employment for Biorem
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Rhodotorula mucilaginosa Isolated from the Manganese Mine Water in Minas Gerais, Brazil: Potential Employment for Bioremediation of Contaminated Water France Anne Dias Ruas & Soraya Sander Amorim & Versiane Albis Leão & Renata Guerra-Sá
Received: 16 July 2020 / Accepted: 2 October 2020 # Springer Nature Switzerland AG 2020
Abstract Manganese (Mn) is an essential heavy metal for living organisms. However, they tend to accumulate the excess of this element causing health problems. The increase of the environmental contamination by this heavy metal is mainly due to mining practices. This work aimed to isolate yeast from mining waters to evaluate its ability of removing Mn2+ ions for the development of further biotechnological and bioremediation applications. The growing interest of this study is
France Anne Dias Ruas and Soraya Sander Amorim contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11270-020-04896-1) contains supplementary material, which is available to authorized users. F. A. D. Ruas : S. S. Amorim : R. Guerra-Sá (*) Laboratório de Bioquímica e Biologia Molecular, Departamento de Ciências Biológicas (DECBI), Instituto de Ciências Exatas e Biológica (NUPEB), Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil e-mail: [email protected] V. A. Leão Laboratório de Bio&Hidrometalurgia, Departamento de Engenharia Metalúrgica e de Materiais (DEMET), Escola de Minas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil
Present Address: R. Guerra-Sá Laboratório de Bioquímica e Biologia Molecular, Departamento de Ciências Biológicas (DECBI), Instituto de Ciências Exatas e Biológica (NUPEB), Campus Morro do Cruzeiro, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil
because it could be effective in the biological treatment of contaminated water, which remains a major challenge for industry and environment. Yeasts were initially isolated from mining water and grown in YPD medium containing 1 to 54 mM Mn2+. Subsequently, the isolates were characterized biochemically and phylogenetically. Then, we evaluate the ability of the isolates of removing Mn2+ ion by SEM scanning electron microscopy coupled with SEM/EDX. Our results showed yeast growth up to 32 mM. There was no pH increase along the tests, suggesting a biological Mn2+ removal. Taken together, the morphological changes in the colony and the darkening of the culture medium suggest the yeast’s ability of oxidizing Mn2+. Five isolates remove these ions considerably, identified as Rhodotorula mucilaginosa. SEM/EDX analysis shows the ability to oxidize and adsorb Mn. The data obtained in this work allows us to conclude that R. mucilaginosa has an important role in the biogeochemical cycle of manganese and present potential biotechnological applications for bioremediation of water contaminated with Mn2+ ions. Keywords Rhodotorula mucilaginosa . Heavy metal . Removal manganese . Bioremediation Abbreviations EDS Microanalysis by energy dispersive spectrometry EDX Energy
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