Larkinella punicea sp. nov., isolated from manganese mine soil
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ORIGINAL PAPER
Larkinella punicea sp. nov., isolated from manganese mine soil Zijie Zhou1 · Lin Zhu1 · Yixuan Dong1 · Xian Xia1,2 · Shijuan Wu1 · Gejiao Wang1 Received: 18 December 2019 / Revised: 11 March 2020 / Accepted: 12 March 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Strain ZZJ9T is a Gram-stain-negative, rod-shaped, aerobic bacterium isolated from manganese mine soil. Strain ZZJ9T showed the highest 16S rRNA gene sequence similarities with Larkinella rosea 15J16-1T3AT (97.1%), Larkinella terrae 15J8-8T (97.0%), Larkinella knui 15J6-3T6T (96.8%), and Larkinella ripae 15J11-1T (95.3%). The genome size of strain ZZJ9T was 8.01 Mb and the DNA G+C content was 51.8 mol%. ANI values among strain ZZJ9T and Larkinella rosea 52004 T, Larkinella knui KCTC 42998T, and Larkinella terrae 52001T were 80.5%, 82.7%, and 80.5%, respectively. dDDH values among strain ZZJ9T and Larkinella rosea 52004T, Larkinella knui KCTC 42998T, and Larkinella terrae 52001T were 23.5%, 26.0%, and 23.6%, respectively. Furthermore, the genome of strain ZZJ9T contained 6302 predicted protein-coding genes and 3114 (49%) of them had classificatory functions. The major quinone of strain ZZJ9T was menaquinone-7 and the main cellular fatty acids were C16:1ω5c (39.5%), iso-C15:0 (25.6%), and iso-C17:0 3OH (11.5%). The polar lipids of strain ZZJ9T were phosphatidylethanolamine, unidentified lipid, and two unidentified aminolipids. Based on the results of phylogenetic, genome, phenotypic, and chemotaxonomic analytical, strain Z ZJ9T represents a novel species of the genus Larkinella, for which the name Larkinella punicea sp. Nov. is proposed. The type strain is Z ZJ9T (= KCTC 62876T = CCTCC AB 2018215T). Keywords Larkinella punicea · Taxonomy · Manganese mine soil
Introduction Communicated by Erko Stackebrandt. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain ZZJ9T is MH817486. The draft genome sequences of strains ZZJ9T, Larkinella rosea KCTC 52004T, Larkinella knui KCTC 42998T and Larkinella terrae KCTC 52001T are QOWE00000000, RQJO00000000, RQJP00000000 and WJXZ00000000, respectively. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01863-6) contains supplementary material, which is available to authorized users. * Gejiao Wang [email protected] 1
State Key Laboratory of Agricultural Microbiology and College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, People’s Republic of China
Hubei Key Laboratory of Edible Wild Plants Conservation & Utilization, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, College of Life Sciences, Hubei Normal University, Huangshi 435002, People’s Republic of China
2
Larkinella is a genus of Cytophagaceae in Bacteroidetes and the type species is Larkinella insperata (Vancanneyt et al. 2006; Anandham et al. 2011). So far, the genus Larkinella comprises nine
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