Aureimonas fodinaquatilis sp. nov., isolated from coal mine wastewater
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ORIGINAL PAPER
Aureimonas fodinaquatilis sp. nov., isolated from coal mine wastewater Jihye Baek1 · Jong‑Hwa Kim1 · Jung‑Sook Lee2 · Ampaitip Sukhoom3 · Wonyong Kim1 Received: 4 March 2020 / Revised: 9 July 2020 / Accepted: 14 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A Gram stain-negative, aerobic, non-motile, short, rod-shaped bacterial strain CAU 1 482T was isolated from coal mine wastewater in Hongcheon, Korea. It grew well at 30 °C, pH 8.5, 2% NaCl (w/v). 16S rRNA-based phylogeny indicated that CAU 1482T forms a distinct lineage within Aureimonas with high similarity to Aureimonas frigidaquae CW5T (98.2%), Aureimonas altamirensis S21BT (98.0%), and Aureimonas glaciei B5-2T (96.3%). The predominant cellular fatty acids were C18:1 2-OH, C 16:0, C18:1 ω7c, and/or C 18:1 ω6c (summed feature 8), with Q-10 as the major isoprenoid quinone. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified aminolipids, and three unidentified lipids. The 3.9-Mb genome included 8 contigs and 3599 protein-coding genes with a 56.7 mol% G + C content. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain CAU 1482T and closely related strains of A. frigidaquae CW5T and A. altamirensis S21BT were 72.2‒72.4% and 18.7‒18.8%, respectively. These phenotypic, chemotaxonomic, and phylogenetic data support CAU 1 482T as a novel Aureimonas species, for which the name Aureimonas fodinaquatilis sp. nov. is proposed. The type strain is CAU 1482T (= KCTC 62995T = NBRC 113692T). Keywords Aureimonas fodinaquatilis · Aurantimonadaceae · Coal mine · Wastewater · Genome · 16S rRNA · Novel species
Introduction The genus Aureimonas in the family Aurantimonadaceae was proposed by Rathsack et al. (2011). Aurantimonas altamirensis, Aurantimonas ureilytica and Aurantimonas frigidaquae of the genus Aurantimonas were transferred as Aureimonas altamirensis, Aureimonas ureilytica and Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01988-8) contains supplementary material, which is available to authorized users. * Wonyong Kim [email protected] 1
Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea
2
Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Republic of Korea
3
Department of Microbiology, Faculty of Science, Prince of Songkla University, Songkhla, Thailand
Aureimonas frigidaquae. At the time of writing, the genus currently comprises 14 species with validly published names (https://www.lpsn.dsmz.de/genus/aureimonas), which have been isolated from various sources, including a water-cooling system (Kim et al. 2008; Rathsack et al. 2011), Altamira Cave (Jurado et al. 2006; Rathsack et al. 2011), a rusty iron plate (Lin et al. 2013), an air (Weon et al. 2007; Rathsa
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