Rickettsia parkeri (Rickettsiales: Rickettsiaceae) detected in Amblyomma maculatum ticks collected on dogs in Tabasco, M
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Rickettsia parkeri (Rickettsiales: Rickettsiaceae) detected in Amblyomma maculatum ticks collected on dogs in Tabasco, Mexico Oswaldo M. Torres‑Chable1 · Bertha G. Jimenez‑Delgadillo2 · Yessenia N. Alvarado‑Kantún3 · Claudia V. Zaragoza‑Vera1 · Guadalupe Arjona‑Jimenez1 · Maritza Zaragoza‑Vera1 · Carlos M. Baak‑Baak3 · Nohemi Cigarroa‑Toledo4 · Ligia Guadalupe Brito‑Argaez5 · Carlos Machain‑Williams3 · Julián E. Garcia‑Rejon3 Received: 17 February 2020 / Accepted: 4 July 2020 © Springer Nature Switzerland AG 2020
Abstract The present study was carried out to identify Rickettsia species with zoonotic potential in ticks collected from dogs in a rural area in Tabasco, Mexico. In total 197 Amblyomma maculatum ticks were collected from 40 domestic dogs. The collected specimens were pooled and subjected to DNA extraction. A fragment (380 bp) of citrate synthase gene (gltA) was amplified by polymerase chain reaction (PCR) using universal primers for Rickettsia. A second PCR was later performed to amplify a fragment (420 bp) of the outer membrane protein B gene (ompB). The PCR products were purified, sequenced and compared using the basic local alignment search tool (BLAST). Twenty out of 40 (50%) tick pools assayed were positive for rickettsial DNA using both primer pairs. The consensus sequence obtained from the ompB gene fragments showed 99.5–100% of identity with strains of Rickettsia parkeri. This study provides the first molecular evidence of the presence of R. parkeri in A. maculatum ticks infesting domestic dogs from southeastern Mexico. Close contact between dogs and humans should lead to consider the infection caused by this species of Rickettsia among the differential diagnoses for people of Tabasco, Mexico, who show acute febrile syndrome associated to inoculation eschar and have a clinical history of tick exposure. Keywords Spotted fever rickettsiosis · Tick-borne rickettsiae · Zoonotic diseases
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s1049 3-020-00524-z) contains supplementary material, which is available to authorized users. * Oswaldo M. Torres‑Chable [email protected] Extended author information available on the last page of the article
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Experimental and Applied Acarology
Introduction Rickettsia parkeri (Rickettsiales: Rickettsiaceae) is classified into the spotted fever group (SFG) Rickettsia species. This bacterium was first reported in 1937 in the Gulf Coast tick Amblyomma maculatum Koch (Parker et al. 1939). The importance of R. parkeri increased after the first report of human infection in the USA (Paddock et al. 2004). At present, in the Americas, R. parkeri encompass four strains, of which two (sensu stricto [s.s.] and Atlantic rainforest) are recognized as human pathogens in the USA, Brazil, Argentina and Uruguay, related to ticks of A. maculatum complex (A. maculatum, A. triste and A. tigrinum) and A. ovale complex (A. ovale and A. aureolatum) as vectors (Nieri-Bastos et al. 2018). In humans, R. parke
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