Role of Acetyl Salicylic Acid in Controlling the DOCA-Salt Induced Hypertension in Rats by Stimulating the Synthesis of
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ORIGINAL ARTICLE
Role of Acetyl Salicylic Acid in Controlling the DOCA-Salt Induced Hypertension in Rats by Stimulating the Synthesis of r-Cortexin in the Kidney Uttam Kumar Maji1,2 • Pradipta Jana3 • Mitali Chatterjee2 • Sanmay Karmakar4 Arup Saha4 • Tamal Kanti Ghosh1,5
•
Received: 2 September 2017 / Accepted: 16 November 2017 Ó Springer International Publishing AG, part of Springer Nature 2018
Abstract Introduction Hypertension is a metabolic disease which is caused by vasoconstriction and that results into elevated blood pressure. A chronic hypertensive condition affects and even damages to various systems in the body. Presence of renal cortexin (r-cortexin), an antihypertensive protein, which is released from the kidney cortex controls the blood pressure. The effect of r-cortexin was mediated through nitric oxide (NO), a universal vasodilating agent. Aim In our study, acetyl salicylic acid (aspirin), a wellknown activator of the endothelial nitric oxide synthase (eNOS) induced r-cortexin synthesis. Methods The hypertensive rat model was prepared by injecting deoxy corticosterone acetate (DOCA). Synthesis of r-cortexin was measured by the anti-r-cortexin antibody which was raised in adult white Wister albino rat model. NO level was determined by using methemoglobin method and later confirmed by chemiluminescence method. Change in blood pressure was determined indirectly by using NIBP monitoring system. Results Aspirin increased the r-cortexin expression from 64.36 ± 12.6 nM to 216.7 ± 21.31 nM in DOCA induced & Tamal Kanti Ghosh [email protected] 1
Department of Pathology, IPGME&R, Kolkata 700020, India
2
Department of Pharmacology, UCM, IPGME&R, Kolkata 700020, India
3
Sinha Institute of Medical Science and Technology, Kolkata 700084, India
4
Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India
5
Department of Health and Family Welfare, Goverment of West Bengal, Kolkata, India
hypertensive rats. The mechanism was proved with the findings of increased level of NO from 0.4 to 1.9 lM. The DOCA induced blood pressure was also decreased from 139.39 ± 7.36 mm of Hg to 116.57 ± 6.89 mm of Hg in case of systolic blood pressure and in case of diastolic pressure from 110.41 ± 7 mm of Hg to 86.4 ± 2.76 mm of Hg that are quite approximate. Conclusion So, from this study it has been found that aspirin induces the r-cortexin synthesis in kidney cortex through the activation of eNOS in DOCA induced hypertensive rats. Keywords Hypertension r-cortexin Nitric oxide Aspirin
1 Introduction As the circulation of blood takes major role to transport food, O2 and minerals so maintenance of blood pressure is very much important. Blood vessels develop a tension against the peripheral resistance. Constriction and dilation in blood vessels play major role in the homeostasis of blood pressure in the system. Though both mechanisms are obligatory, but retention of any state of these two in the circulatory system may cause life-risk [1]. Hypertension, which is caused by constriction of
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