Screening of Surface-associated Bacteria from the Mexican Red Alga Halymenia floresii for Quorum Sensing Activity
- PDF / 825,424 Bytes
- 11 Pages / 612 x 792 pts (letter) Page_size
- 15 Downloads / 208 Views
XPERIMENTAL ARTICLES
Screening of Surface-Associated Bacteria from the Mexican Red Alga Halymenia floresii for Quorum Sensing Activity S. A. Abdul Malika, *, A. Bazirea, A. Gamboa-Muñozc, G. Bedouxa, D. Robledob, J. Q. García-Maldonadod, and N. Bourgougnona aUniversité
Bretagne Sud, EA 3884, LBCM, IUEM, Vannes, F-56000 France Marine Resources Department, CINVESTAV-Unidad Cordemex, Merida, 97310 Mexico c Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Unidad Mérida, Departamento de Recursos del Mar (CINVESTAV-Unidad) Cordemex, Merida, 97310 Mexico d CONACYT−CINVESTAV Marine Resources Department Cordemex Merida, 97310 Mexico *e-mail: [email protected] b
Received April 8, 2020; revised July 15, 2020; accepted July 21, 2020
Abstract—Macroalgae host a dense bacterial epibiome forming surface biofilms, which act as a biological defense by protecting the surface from macrofoulers. During experimental cultivation of Halymenia floresii (Rhodophyta, Halymeniales) under Integrated MultiTrophic Aquaculture (IMTA), the culture tanks remained clean of any exogenous algal colonization, and the surface of the H. floresii thalli was remarkably free from any fouling organisms. The presence of Halymenia also appeared to restrict the establishment of opportunist green algae and the colonization of barnacles usually impairing the cultures. To date, nothing is known about the diversity and biological potential of H. floresii epibionts. Hence, to better understand their epiphytic bacterial community, surface-associated bacteria from distinct H. floresii samples, beach-cast, integrated multi trophic aquaculture cultivar, and cultivar cylinders, were isolated. Thirty-one axenic bacterial strains belonging to 3 phyla, 20 genera, and 25 species were identified by 16S rRNA gene sequencing. Following isolation, they were screened for the production of quorum sensing (QS) signals, since these communication signals play a vital role in biofilm formation. All, except one, isolates were identified as gram-negative; hence, acylated homoserine lactones (AHLs) were focused upon. Using the reporter strain Escherichia coli pSB406, the isolates were screened for their ability to induce luminescence. Of 31 isolates screened, 17 strains, including Pseudoalteromonas arabiensis, Pseudoalteromonas sp. (B5BC and B6.1BC), Pseudoalteromonas mariniglutinosa, Vibrio owensii, Tenacibaculum sp., Maribacter sp., Spongiimicrobium salis, Aquimarina sp., Kordiimonas sp., Alteromonas sp. (B12CC and B16CC), Roseobacter sp., Erythrobacter sp., Ruegeria lacuscaerulensis, and Epibacterium sp., exhibited the presence of extracellular QS signals. Thus, the presence of QS bacteria on the surface of H. floresii was demonstrated for the first time. Our results may open a new direction to investigate the host—QS bacteria interactions. Keywords: Halymenia floresii, surface-associated bacteria, quorum sensing, AHLs, 16S rRNA DOI: 10.1134/S0026261720060132
Macroalgae and their associated microbiota form a functional
Data Loading...
