Semi reflective biopolymer layers for the detection of biomass hydrolytic enzymatic activities
- PDF / 830,006 Bytes
- 6 Pages / 612 x 792 pts (letter) Page_size
- 7 Downloads / 168 Views
Semi reflective biopolymer layers for the detection of biomass hydrolytic enzymatic activities C. Cerclier, C. Moreau, A. Guyomard-Lack, E. Bonnin, H. Bizot and B. Cathala, UR1268 Biopolymères, Interactions et Assemblages, 44300 Nantes, France ABSTRACT Structural colors were obtained by the deposition of plant cell walls biopolymers films on reflective support. Multilayered xyloglucan(XG)/cellulose nanocrystals(CN) thin films were obtained by spin-assisted layer-by-layer assembly while arabinoxylan (AX) thin films were elaborated via the spin-coating of AX/melamine formaldehyde resin followed by a cross-linking step. The effects of aqueous solutions on the stability of the structural colors were evaluated. The films were subsequently used to detect cellulase and xylanase activities by the change in the colors due to the film degradation. This enzymatic assay method appeared to be about 150 more sensitive that a standard method. Moreover due its simplicity, the method could be used to detect other biomass-hydrolyzing enzymes and more generally for other heterocatalytic degradations of solid polymer layers. INTRODUCTION Plant biomass is an abundant and renewable resource that can be used for the production of fuel ethanol and industrially-relevant chemicals. However, the use of this raw material via integrated biorefineries plants is a considerable technological challenge, particularly because of its chemical complexity and its recalcitrance to classical cracking strategies. Indeed, plants cell walls are complex and intricate materials consisting in many polymer families among which cellulose and xylan are the most abundant polysaccharides. The structural heterogeneity and complexity of plant cell wall constituents induce a high recalcitrance to degradation processes and require combination of processes and catalysts. Among the technologies considered to overcome these difficulties, enzymatic bioconversion offers an environment-friendly alternative. The use of enzymes needs a constant effort for discovering new heterocatalytic activities in order to improve biomass fractionation and transformation. Beside genetic evolution of known enzymes, systematic screening of various and complex biological media is also a promising approach. However, the prerequisite for an efficient screening of a large sample set is the availability of simple to handle, reliable and rapid tests. Deposition of a thin layer at the surface of a substrate can induce the appearance of interferential colors when the thickness of the layer is tuned to an appropriate subwavelength value [1, 2]. These colors are due to constructive or destructive interferences between white light components reflected from the air-film interface and from the film-substrate interface. We present here the elaboration of a semi-reflective biopolymer layer on a silicon wafer. Semireflective layers are commonly obtained by mono or multilayers deposition either by dipping or spin-coating [2, 3-5]. When such layers are in presence of enzymes able to hydrolyze the constitutive b
Data Loading...
