Antioxidant enzyme regulating and intracellular ROS scavenging capacities of two novel bioactive peptides from white gru
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Medicinal Chemistry Research (2020) 29:2039–2049 https://doi.org/10.1007/s00044-020-02623-3
ORIGINAL RESEARCH
Antioxidant enzyme regulating and intracellular ROS scavenging capacities of two novel bioactive peptides from white grub larvae (Polyphylla adstpersa) hydrolysate in A549 cells Asal Khajepour-Zaveh1 Ahmad Asoodeh ●
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Hossein Naderi-Manesh1
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Received: 28 April 2020 / Accepted: 27 August 2020 / Published online: 8 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract This study is aimed at extracting and purifying bioactive peptides from white grub larvae (Polyphylla adspersa) hydrolysate and examining their antioxidant effects on lung cancer cells (A549). Bioactive peptides from white grub larvae hydrolysate (WGLH) were produced using papain, pancreatin, proteinase-K and trypsin. The results showed that papain hydrolysate had the most 1, 1-diphenyl-2-pycryl-hydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging. This hydrolysate was fractionated by reverse-phase liquid chromatography (HPLC) and two most antioxidant fractions were identified by tandem mass spectrometry that had the sequence of YPQSLRWRAK (1304.6 Da, hereby called YK-10) and LPLFFYDVRP (1266.6 Da, hereby called LP-10). Cytotoxicity effect of YK-10 and LP-10 peptides on A549 cells, human umbilical vein endothelial cells (HUVECs) and human red blood cells were examined. The two peptides induced the specific activity of catalase, superoxide dismutase, glutathione peroxidase, and total level of thiol in A549 cells. Reactive oxygen species in treated A549 cells were significantly lower than that of controls. In conclusion, our results suggest that YK-10 and LP-10 peptides from WGLH have antioxidant activities and can protect A549 cells against free radicals’ damages. Keywords Polyphylla adspersa Peptide Cell protection Antioxidant enzymes A549 cells ●
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Introduction Insects are the most successful group of animals on the earth, having economic importance and Coleoptera or beetles are the most diverse insect order (Rader et al. 2016). The Scarabaeidae with 27,800 species are one of the most toxic and dangerous polyphagous pests of forest trees, ornamental plants, and plants (Seabrooks and Hu 2017). The subfamily of Melolonthidae with 750 genera and 11,000 species is
* Ahmad Asoodeh [email protected] * Hossein Naderi-Manesh [email protected] 1
Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
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Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
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Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
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recorded worldwide with a population of more than one-third of the species of this family (Faruck et al. 2016; Seabrooks and Hu 2017). Polyphylla adspersa and Polyphylla olivieri species are the most economically important pests (Demir et al. 2015; Karimi and Salari 2015). P. adspersa is limited
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