Sequence analyses of RT-PCR products obtained from seven infected leaf samples revealed existence of three potyvirus spe
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ORIGINAL ARTICLE
Sequence analyses of RT‑PCR products obtained from seven infected leaf samples revealed existence of three potyvirus species in Indian narcissus (Narcissus tazetta L.) Rashmi Raj1,2 · Charanjeet Kaur1 · Ashish Srivastava1,3 · Susheel Kumar1 · S. K. Raj1 Received: 27 May 2020 / Accepted: 29 August 2020 © King Abdulaziz City for Science and Technology 2020
Abstract Potyvirus species associated with yellow leaf stripe disease of Indian narcissus (Narcissus tazetta L.) var. Paperwhite has been studied by sequence analyses of ~ 1.5 kb genomic fragments obtained from seven RT-PCR amplifications of infected samples. Sequence analysis revealed the occurrence of three potyvirus species: cyrtanthus elatus virus-A (CEVA: KF430815, KF430816, KM066973, KM066974); narcissus yellow stripe virus (NYSV: KM066972, JQ686724) and narcissus degeneration virus (NDV: MK572806). The existence of three potyvirus species: CEVA, NYSV and NDV are being reported in Indian narcissus. Keywords N. tazetta var. paperwhite · Leaf yellow stripe · RT-PCR amplification · Sequence identity · Phylogenetic relationships · CEVA · NYSV · NDV
Introduction Indian narcissus (Narcissus tazetta L.) of the family Amaryllidaceae is a bulbous ornamental plant. It is popular for its beautiful flowers in the garden beds and used as a cut-flower for bouquets, vases and also for the production of fragrant oil and perfumes in India. N. tazetta is common ornamental species in the Mediterranean region (from Portugal to Turkey), considered as its native place, though, its dissemination extended throughout the Asian countries with demand-based international trading (Harvey and Selby 1997; Hanks 2002; Kamenetsky and Okubo 2012). Now, N. tazetta are widely grown in China, Israel, India, and Japan, and the large volumes of field-grown cut-flowers are traded, along with other commercially important flowers and pot-grown plants as well as the bulbs (Hank and Chastagner 2018). * S. K. Raj [email protected] 1
Plant Virus Laboratory, CSIR-National Botanical Research Institute, Lucknow 226001, India
2
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, UP 201002, India
3
Amity Institute of Virology and Immunology, Amity University Uttar Pradesh, Noida, UP 201313, India
Narcissus are reported worldwide to be infected by narcissus mosaic virus (NMV), narcissus yellow stripe virus (NYSV), narcissus late season yellow virus (NLSYV), narcissus degeneration virus (NDV), narcissus latent virus (NLV), narcissus tip necrosis virus (NTNV), cyrtanthus elatus virus-A (CEVA) and ornithogalum mosaic virus (OrMV) causing streaks, yellow stripe and tip necrosis symptoms on the leaf (Brunt 1977, 2008; Wylie and Jones 2012). Other viruses such as raspberry ring spot virus (RRSV), nerine latent virus (NeLV), narcissus symptomless virus (NSV), arabis mosaic virus (ArMV), cucumber mosaic virus (CMV), tobacco rattle virus (TRV) and tomato black ring virus (TBRV) are also reported to infect narcissus though their frequency of infection is reported co
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