Silver nanoparticles induce genetic, biochemical, and phenotype variation in chrysanthemum
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ORIGINAL ARTICLE
Silver nanoparticles induce genetic, biochemical, and phenotype variation in chrysanthemum Alicja Tymoszuk1 · Dariusz Kulus1 Received: 21 May 2020 / Accepted: 19 August 2020 © The Author(s) 2020
Abstract Despite the tremendous progress in breeding, novel and user-friendly techniques of plant improvement are desirable. The study aimed to analyze the usefulness of silver nanoparticles (AgNPs) in the breeding of chrysanthemum: one of the top ornamental plant species. In vitro regeneration of adventitious shoots from internodes of chrysanthemum ‘Lilac Wonder’ was induced on the modified Murashige and Skoog (MS) medium supplemented with 0.6 mg L −1 6-benzylaminopurine −1 (BAP), 2 mg L indole-3-acetic acid (IAA) and AgNPs at 0, 5, 10 and 20 ppm concentration. The efficiency of callogenesis and caulogenesis were analyzed after 10 weeks of culture. The concentration of chlorophylls, carotenoids, and phenolic compounds in shoots and calli were estimated. Plants obtained from 20 ppm AgNPs treatment were additionally analyzed on the genetic level using randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. In vitro rooted shoots were acclimatized in the glasshouse and subjected to biochemical and phenotype stability evaluation. AgNPs at the highest concentration (20 ppm) suppressed both callogenesis and caulogenesis in vitro. The concentration of metabolites in callus was stable, regardless of AgNPs treatment, except for carotenoids which production was enhanced by 20 ppm AgNPs. In contrast, the content of chlorophyll a and b in shoots varied depending on AgNPs treatment. Polymorphic loci were detected in 12 and 9 AgNPs-treated-plants by RAPD and ISSR markers, respectively (one of which was common to both marker systems). Rooting and acclimatization were fully successful in all experimental combinations. Phenotype alternations were detected in six plants; one from 10 ppm AgNPs treatment and five from 20 ppm treatment. They included variation in pigment content (anthocyanins and carotenoids) and/or inflorescence shape. Interestingly, only two plants revealed both genetic and phenotype polymorphisms. No genetic or phenotype variation was detected in the control plants. In conclusion, AgNPs can be used in chrysanthemum breeding. Key message Silver nanoparticles added to the culture medium affect the regeneration and metabolism of plants. Moreover, they may be a source of significant genetic and phenotype variation in the in vitro-propagated chrysanthemum. Keywords Chrysanthemum × grandiflorum · ISSR · Metabolites · Mutagenesis · Nanotechnology · RAPD
Introduction
Communicated by M. I. Beruto. * Alicja Tymoszuk [email protected] 1
Laboratory of Ornamental Plants and Vegetable Crops, Faculty of Agriculture and Biotechnology, UTP University of Science and Technology in Bydgoszcz, 6 Bernardyńska Str., 85‑029 Bydgoszcz, Poland
Chrysanthemum × grandiflorum /Ramat./Kitam. is one of the most valuable and popular ornamental plant species in the horticultural
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