Simple sequence repeat marker based genetic diversity assessment amongst high essential oil yielding lines of Curcuma ca
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RESEARCH ARTICLE
Simple sequence repeat marker based genetic diversity assessment amongst high essential oil yielding lines of Curcuma caesia Roxb. Manabi Paw . Angana Borah . Sudin Kumar Pandey . Joyashree Baruah . Twahira Begum . Mohan Lal
Received: 19 February 2020 / Accepted: 18 November 2020 Ó Springer Nature B.V. 2020
Abstract Curcuma caesia Roxb. belonging to Zingiberaceae family is a rhizomatous perennial herb consisting of bluish black rhizome and having vast medicinal and pharmaceutical applications. It is considered as an endangered medicinal plant and native to India. The aim of this study is to assess the molecular diversity among the high rhizome essential oil yielding lines of C. caesia ([ 1.5% w/w). The molecular diversity analysis was done by using 45 simple sequence repeat markers amongst the 78 high essential oil yielding lines of C. caesia. The overall percentage of polymorphism amongst the lines was found to be 53%. AMOVA analysis showed 20% and 80% variations among and within the population respectively. Dendrogram constructed using Unweighted Pair Group with Arithmetic Mean (UPGMA) for those high essential oil yielding lines sorted into five major clusters according to their different geographical locations. These five clusters were confirmed using principle component analysis
M. Paw S. K. Pandey J. Baruah T. Begum AcSIR- Academy of Scientific and Innovative Research, Ghaziabad 201002, India M. Paw A. Borah S. K. Pandey J. Baruah T. Begum M. Lal (&) Medicinal, Aromatic and Economic Plants Group, Biological Science and Technology Division, CSIR-North East Institute of Science and Technology (NEIST), Jorhat, Assam 785006, India e-mail: [email protected]; [email protected]
(PCA), based on the Jaccard’s similarity coefficient. The results revealed that these microsatellite markers could be used as successful tool to differentiate the genetic makeup of the crop. Molecular diversity of C. caesia has not been studied till now. So, this is the first scientific report on molecular diversity analysis which may help in the further crop improvement programme and germplasm conservation also. Keywords Curcuma caesia Cluster analysis High essential oil yield Molecular variability Simple sequence repeat marker
Introduction The genus Curcuma belonging to Zingiberaceae family, contains about 92 species of perennial rhizomatous herbs (The Plant List 2013; Pandey and Gupta 2014). These herbs are found in Asian pacific regions, of which 50% are found to be native of India (Liu et al. 2013). These species are well-known for their medicinal and therapeutic properties (Liu et al. 2013). The genus Curcuma contains one of the important species namely Curcuma caesia Roxb. which is an endangered plant native to the North Eastern part of India which is also known as Kali Haldi (Paliwal et al. 2011; Paw et al. 2020; Borah et al. 2019; Sharma et al.
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Genet Resour Crop Evol
2017). This economically important species is widely distribut
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