Evaluating Genetic Diversity Within Genus Jasminum L. (Oleaceae) Using Intersimple Sequence Repeats (ISSR) Marker
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RESEARCH ARTICLE
Evaluating Genetic Diversity Within Genus Jasminum L. (Oleaceae) Using Intersimple Sequence Repeats (ISSR) Marker Regy Yohanan1 • Nirmala J. Jeyarani2 • V. Devipriya3 • Shabir A. Rather4 Shruti Kasana4 • Julie Thakur4 • Mayank D. Dwivedi4 • Arun K. Pandey4
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Received: 2 August 2018 / Revised: 26 June 2019 / Accepted: 13 July 2019 The National Academy of Sciences, India 2019
Abstract Jasmines are an important group of plants extensively used in the perfumery industry, preparation of garland and for ornamental purposes. To analyze the genetic potential of the group, the authors evaluated its genetic diversity. Intersimple sequence repeats (ISSR) markers were used to evaluate genetic diversity among 40 accessions of 23 Jasminum species including six endemic species. The present study is the first report of the efficacy of 10 shortlisted ISSR markers out of 30 primers screened. Among 23 Jasminum species, J. bignoniaceum revealed maximum genetic diversity and Shannon’s Index. Results revealed that the accessions of Jasminum species are grouped into three major clades, showing close agreement with the morphological relatedness. The diversity analysis also showed that the members of section Alternifolia are the ancestor to the rest of the Jasminum spp. The study provides direction for the future population study on Jasminum spp. This is the first report on the genetic diversity Significance Statement Genus Jasminum is an economically important oil-yielding taxa. Despite its high economic value, no molecular study was done to assess the genetic diversity within the genus in India. ISSR-PCR technique will help to estimate the genetic similarities and evaluate relationships between available accessions of Jasminum species in India. & Arun K. Pandey [email protected] 1
Department of Botany, Sree Narayana College, Kollam, Kerala, India
2
Department of Botany, Fatima Mata National College (Autonomous), Kollam, Kerala, India
3
Department of Botany, Sree Narayana College, Chempazhanthy, Thiruvananthapuram, Kerala, India
4
Department of Botany, University of Delhi, Delhi 110007, India
assessment of Indian Jasminum spp. incorporating approximately 50% species. Keywords Genetic diversity Intersimple sequence repeats (ISSR) Jasmines
Introduction The genus Jasminum L. (Oleaceae) includes approximately 200 species, distributed in the tropical and warm temperate regions of the Old World [1, 2]. In India, the genus is represented by 47 species, three subspecies and four varieties, of which 16 species are endemic. The majority of the endemic species have been reported from Eastern and Western Himalaya, Deccan Peninsula, and Andaman and Nicobar Islands [3]. The genus has been subdivided into five sections viz. Unifoliolata, Alternifolia, Jasminum, Trifoliolata, and Primulina [4, 5]. There is much ambiguity within the sectional classification owing to frequent overlapping of the species boundaries as well as phenotypic plasticity arising from the extensive reticulation of exomorphological features (F
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