Simultaneous Determination of Erythromycin, Tetracycline, and Chloramphenicol Residue in Raw Milk by Molecularly Imprint
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Simultaneous Determination of Erythromycin, Tetracycline, and Chloramphenicol Residue in Raw Milk by Molecularly Imprinted Polymer Mixed with Solid-Phase Extraction Yunfei Xie 1,2,3 & Qi Hu 1,2,3 & Mengyao Zhao 1,2,3 & Yuliang Cheng 1,2,3 & Yahui Guo 1,2,3 & He Qian 1,2,3 & Weirong Yao 1,2,3
Received: 13 April 2017 / Accepted: 20 July 2017 # Springer Science+Business Media, LLC 2017
Abstract A rapid, simple, and efficient method was described for the simultaneous determination of three common antibiotic residues including erythromycin, tetracycline, and chloramphenicol in raw milk. A molecular imprinting technique combined with solid-phase extraction (SPE) was used to pretreat the test samples and then simultaneously detected with HPLC. The whole process, only including one step of pretreatment, was able to detect the entire target molecule using specific enrichment and analysis method. The detection was verified by comparison with Chinese standard methods of GB/T 229882008, GB/T 22990-2008, and GB/T 29688-2013. The testing lines, accuracy, reproducibility, specificity, and recoveries were evaluated, and calculated values were in line with established standards. The recoveries of erythromycin, tetracycline, and chloramphenicol were 77.82~87.08, 81.02~88.17, and 72.94~83.57%, respectively. By using of this novel solid-phase extraction substrate, the detection limits for erythromycin, tetracycline, and chloramphenicol were 10, 20, and 10 μg kg−1, respectively. The method was suitable for routine analysis, and the experimental procedure was simplified, with the detection time greatly reduced. This method showed
* Weirong Yao [email protected] 1
State Key Laboratory of Food Science and Technology, Wuxi, Jiangsu 214122, China
2
School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China
3
Collaborative innovationcenter of food safety and quality control in Jiangsu Province, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China
broad application prospects for the detection of antibiotic residues in raw milk. Keywords Simultaneous detection . Molecular imprinting . Solid-phase extraction . Antibiotics . Raw milk
Introduction Antibiotics are important drugs for the prevention and treatment of microbial infections and therefore widely used in human medicine and animal husbandry. Three kinds of antibiotics, chloramphenicol (CAP), erythromycin (EM), and tetracycline (TC) owing to their high oral bioavailability and ability to accumulate intracellularly in many tissues and organs, were widely used in medical and veterinary practices to prevent and treat diseases in swine, poultry, cattle, and sheep. Antibiotic treatment of cows during the breeding process was especially common; further examples of their use in cows include the use of EM and TC to treat clinical or subclinical mastitis (Wang et al. 2006) and the use of CAP to treat bacterial gastroenteritis. Due to the low cost and efficacy of these three antibiotics, irrational use and noncompliance with the withdra
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