Single-cell transcriptional profile of ACE2 in healthy and failing human hearts
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ngle-cell transcriptional profile of ACE2 in healthy and failing human hearts 1,2†
Yitian Zhou 1
2†
1†
2†
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, Yongfa Huang , Xiaomin Song , Xiaoxiao Guo , Junling Pang , Jing Wang , 2* 3 Shuyang Zhang & Chen Wang
State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Department of Pathophysiology, Peking Union Medical College, Beijing 100730, China; Department of Cardiology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical
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Sciences, Beijing 100730, China; State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Department of Physiology, Peking Union Medical College, Beijing 100730, China Received May 20, 2020; accepted August 16, 2020; published online September 1, 2020
Citation:
Zhou, Y., Huang, Y., Song, X., Guo, X., Pang, J., Wang, J., Zhang, S., and Wang, C. (2020). Single-cell transcriptional profile of ACE2 in healthy and failing human hearts. Sci China Life Sci 63, https://doi.org/10.1007/s11427-020-1787-5
Dear Editor, The coronavirus disease 2019 (COVID-19) pandemic, which is caused by SARS-CoV-2, has gained serious attention from medical practitioners around the world in the past few months. Approximately 20% of critically ill COVID-19 patients were reported to have suffered myocardial injury. The specific mechanism of this pathology requires further investigation (Yang et al., 2020). It was reported that SARSCoV-2 invades alveolar epithelial cells via the angiotensin converting enzyme (ACE2) cell receptor in the lungs, but whether cardiomyocytes can be directly infected with SARSCoV-2 via ACE2 remains controversial (Zhou et al., 2020). The rapid development of single-cell RNA sequencing (scRNA-seq) in recent years has enabled us to analyze the transcriptome with a single-cell resolution. In this study, we analyzed the scRNA-seq data from human hearts in the Gene Expression Omnibus public database to investigate the expression profile of ACE2 and the expression profiles of other genes in different types of human heart cells.
†Contributed equally to this work *Corresponding authors (Jing Wang, email: [email protected]; Shuyang Zhang, email: [email protected])
Briefly, the scRNA-seq data from the heart samples of 14 healthy adults and four heart failure patients (GSE109816, GSE121893), as well as those from 20 fetal hearts (GSE106118), were included in the analysis. The Seurat R package (v.3.1.2) was used to perform data integration and clustering analysis to approximately 14,000 single cells (Stuart et al., 2019). The clusters were annotated based on canonical cell-type marker genes (Han et al., 2018; Volz et al., 2015). The main cell types of the heart, including cardiomyocytes and cardiac fibroblasts, were identified in both the hearts of adult humans and the hearts of human embryos (Figure 1A, B, and H). Consistent with the results of previous studies analyzing the expression levels of
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