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ORIGINAL PAPER

Two Coordination Polymers: Protective Activity On Nerve Injury by Increasing miR-219 and Reducing Inflammatory Response Guan-Dong Li1 • Sui-Pian Lai1 • Bing-Hua Li1 • Yi-An Zhan2 • Fen Liu2 Received: 27 June 2020 / Accepted: 19 September 2020  Springer Science+Business Media, LLC, part of Springer Nature 2020

Abstract Two novel transition metal coordination polymers (CPs) of {Cu(tdc)(Hdmea)2 (1, Hdmea = N,N-dimethylethanolamine, tdc = 2,5-thiopenedicarboxylate) and [Co3(H2O)3(tdc)3(dioxane)]
2(dioxane) (2) were prepared via the reaction of Co(NO3)2
6H2O or Cu(NO3)2
3H2O and H2tdc with or without the template reagent Hdmea. The following experiments were carried out to determine the treatment activity for compounds against nerve injury. Firstly, the real time RT-PCR was carried out to determine the miR-219 relative expression in the nerve cells. Then the activation level of the AMPK signaling pathway in the nerve cells was measured with western blot. In addition to this, the ELISA detection was also used in this research, for the inflammatory cytokines’ evaluation. Finally, the inhibition of the compound 1 and compound 2 on the death of the nerve cells was determined with MTT assay. Keywords Coordination polymer
Nerve injury
miR-219
RT-PCR

Introduction It was previously thought that the brain tissue is unable to produce the inflammatory response to the injury stimulation [1]. However, in recent years, a large number of researches have shown that the central nervous system can produce a complete inflammatory response to various damages. AMPK signaling pathway in the nerve cells was regarded as one of the most important pathways, which adjust the level of inflammatory responses of nerve cells [2]. The activation of signaling pathway AMPK could significantly up-regulate the releasing the IL-b and IL-6. However, the over activated level of the inflammatory response in the nerve cells could also cause damages to the cells, leading to the cells’ death rate. Coordination polymers (CPs) in the previous researches have showed excellent treatment against serious of disease, such as coordination polymers were proved has anti-liver cancer & Guan-Dong Li [email protected] 1

Department of Neurology, Jiangmen Central Hospital, Jiangmen, Guangdong, China

2

Department of Medicine, Nanchang University, Nanchang, Jiangxi, China

activity by reducing the synergistic NO and chemodynamic [3]. Thus, in this present research, the application values of the CPs on the nerve injury clinic treatment was still need to be explored, and various experiment were performed for the evaluation. Coordination polymers (CPs) have unique porous architecture and wide application prospect, and their reasonable preparation method has obtained much attention [4–6]. CPs’ structural diversities are inevitably caused by the extensive selection of building blocks with organic connectors and metal nodes [7–9]. In addition, because of complex synthesis elements for instance coordination of the me