Spectrofluorometric Method Development and Validation for the Determination of Curcumin in Nanoliposomes and Plasma
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ORIGINAL ARTICLE
Spectrofluorometric Method Development and Validation for the Determination of Curcumin in Nanoliposomes and Plasma Maryam Karimi 1,2 & Mohammad Mashreghi 1,2 & Sara Shokooh Saremi 1,2 & Mahmoud Reza Jaafari 1,2,3 Received: 19 March 2020 / Accepted: 23 June 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract In this paper, we have reported a rapid, simple, sensitive, straightforward, and validated method for the concentration determination of curcumin (CUR) in nanoliposomes and plasma using the spectrofluorimetry. For both nanoliposomal formulation and plasma, methanol was used as a solvent to extract the CUR. The excitation and emission wavelengths were set at 423 nm and 527 nm, respectively. The method validation was performed based on International Council for Harmonization (ICH) guidelines, Q2, in which parameters; such as, linearity, precision, accuracy and etc., were determined. The results showed that the calibration curve was linear for CUR concentrations of 0.05 to 0.5 μg /mL with a correlation coefficient of 0.9996. The limit of detection (LOD) and limit of quantification (LOQ) were 0.03 and 0.10 μg/mL, respectively. Liposomal CUR (15 mg/kg) was injected intravenously to mice, and at certain time intervals (1, 3, 6, and 24 h), blood samples were collected. The samples were extracted by methanol and CUR concentrations were detected using a fluorescence spectrophotometer. Results indicated the rate of liposomal formulation decline was slower than free CUR. The results of this study indicated that the validation method based on fluorimetry which was developed here is reliable for the detection of CUR in liposomal formulations and plasma. Keywords Curcumin determination . Fluorimetry . Method validation . Plasma
Introduction Curcumin (CUR), a natural polyphenolic compound extracted from the roots of Curcuma longa, is basically used as a spice in food, meantime used as medicine due to an extensive range of pharmacological activities; such as, antitumor, anti-inflammatory, antioxidant and antimicrobial properties [1, 2]. Although CUR therapeutic properties have been verified, its poor water solubility and low bioavailability has caused problems, restricting its clinical efficacy [3]. Therefore, efforts have been done in order to increase the bioavailability of CUR using carrier system. Nanocarriers are a group of drug delivery system that can be helpful in overcoming such * Mahmoud Reza Jaafari [email protected] 1
Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
2
Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
3
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
problems. Incorporating the drugs into liposomes, micelles, polymeric nanoparticles, and lipid nanocarriers can increase their solubility, bioavailability, and therapeutic efficacy.
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