STIMULATING EFFECTS OF MULTIWALLED CARBON NANOTUBES ON IMMOBILIZED GLUCONACETOBACTER SUCROFERMENTAS VKPM B-11267 CELLS
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CES AND PRODUCTS BASED ON NANOMATERIALS AND NANOTECHNOLOGIES
Stimulating Effects of Multiwalled Carbon Nanotubes on Immobilized Gluconacetobacter sucrofermentas VKPM B-11267 Cells S. E. Tarasova,*, Yu. V. Plekhanovaa, A. G. Bykova, N. A. Klenovab, and A. N. Reshetilova,* a
Skryabin Institute of Biochemistry and Physiology of Microroganisms of the Russian Academy of Sciences (IBPM RAS)— Subdivision of Pushchino Biological Research Center of the Russian Academy of Sciences, Pushchino, Moscow oblast, 142290 Russia b Samara National Research University, Samara, 443086 Russia *e-mail: [email protected] Received May 7, 2020; revised May 7, 2020; accepted May 14, 2020
Abstract—The Gluconacetobacter sucrofermentas bacterial cells are immobilized in a chitosan gel on the surface of an electrode obtained by matrix printing. The nature of the change in the bioelectrochemical parameters of the immobilized bacteria under the influence of multiwalled carbon nanotubes (MWCNTs) are studied. We studied the electrochemical signals of cells upon the introduction of substrates (glucose, ethanol) by three methods: chronoamperometry, cyclic voltammetry, and impedance spectroscopy. The introduction of ethyl alcohol at a concentration of 1 mM leads to a decrease in the total resistance of the electrode by a factor of ~40. Modification of the electrode using MWCNTs leads to an increase in the current (by factors of 8 to 10) and an additional decrease in the total electrode resistance by a factor of ~10. These effects are similar to the effects of substrates and MWCNTs on the phylogenetically related Gluconobacter oxydans cells. The formation of bacterial cellulose (BC) membrane cells during the growth does not fundamentally change the effects of the substrates and MWCNTs. DOI: 10.1134/S1995078020010127
INTRODUCTION Gluconacetobacter sucrofermentas VKPM B-11267 phylogenetically resemble the Gluconobacter oxydans VKM 1280 bacteria [1, 2], which, as shown in [3–5], are an effective object for the formation of microbial biosensors. In this study, we find out how effective the G. sucrofermentas VKPM B-11267 cells can be in the construction of biosensors and whether the presence of nanomaterial affect the behavior of the cells, as well as the role the membrane of this bacterial cells plays and whether it leads to a significant increase in the resistance of the layer of immobilized bacteria and a significant loss of signal. The G. sucrofermentas VKPM B-11267 bacteria are a producer of bacterial cellulose (BC) [6, 7]. BC is a promising material for obtaining a wide range of products and nanomaterials. It has great potential for use in medicine as a biomaterial for tissue engineering and creating wound dressings and transdermal therapeutic systems; it can be used in nutrition as a carrier of additives for balanced nutrition; it can be used in industrial electronics to produce optically transparent compounds with an ultralow coefficient of thermal expansion for manufacturing acoustic diaphragms; and it can serve as a substitute for plant cellulose
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