Surface-functionalized Electrospun Polycaprolactone Fiber for Culturing Stem Cell from Human Exfoliated Deciduous Teeth
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ISSN 1229-9197 (print version) ISSN 1875-0052 (electronic version)
Surface-functionalized Electrospun Polycaprolactone Fiber for Culturing Stem Cell from Human Exfoliated Deciduous Teeth Culture Jirasak Jitpibull1, Thammasit Vongsetskul1,2*, Hathaitip Sritanaudomchai3, and Nathaphon Tangjit4 1
School of Materials Science and Innovation, Faculty of Science, Mahidol University, Nakhon Pathom 73170, Thailand 2 Department of Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand 3 Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand 4 Department of Orthodontics, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand (Received October 23, 2019; Revised February 4, 2020; Accepted February 10, 2020) Abstract: Smooth and bead-free polycaprolactone (PCL) fiber with average diameter of 2.8 micron and pore size of 25 micron were spun from 17 % w/w PCL dissolved in a chloroform:methyl alcohol mixture (3:1 by volume) at 20 kV, a fiber collection distance of 15 cm, a nozzle diameter of 0.9 mm, and 2 ml/h for 5 hours. Then, the fiber surface was immobilized by gelatin (GE), coated by hyaluronic acid (HA), and done by both processes to obtain GE-PCL, HA-PCL, and HA-GE-PCL, respectively. The treated fiber was completely wet by water, whereas the PCL fiber was not. GE improved thermal stability, while HA increased the tensile strength and elastic moduli of the fiber. Both GE and HA reduced their elongations at break. Stem cells from human deciduous teeth (SHED) were chosen to study their proliferation on the fiber, which was exponentially in the following order: GE-PCL > HA-GE-PCL > HA-PCL > PCL. Keywords: Fiber, Gelatin, Human exfoliated deciduous tooth, Polycaprolactone, Tissue engineering
because PCL meets the minimum requirement to be used in fabricating scaffolds such as good mechanical performance [15,16], biocompatibility, and biodegradability [17,18]. In this work, the PCL ultrafine fiber will be produced and their surface will be modified to make it hydrophilic to promote cell proliferation, cell attachment, and cells differentiation because protein is highly absorbed on hydrophilic surface. PCL surface will be either treated chemically or layer-bylayer coated with biopolymers. Hyaluronic acid (HA) is a natural polysaccharide in ECM [19]. HA is composed of two monomers, N-acetyl-Dglucosamine and D-gluconic acid [20]. HA is used in tissue engineering due to its no toxicity, non-immunogenicity, and biodegradability [21]. HA is widely used to treat surface of polymer scaffold to improve surface hydrophilicity and scaffold’s mechanical properties [22,23]. Gelatin (GE), a hydrophilic protein from collagen, is also widely used in tissue engineering to promote cell proliferation [24-28]. GE can be mixed with PCL solution before spinning [29], coated [30], or immobilized [31-33] on scaffold surface. Thus, in this work, we developed electrospun PCL fiberbased scaffolds which were treated by GE, HA, and both to improve their biocompatibilities with stem cells
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