Effect of ciliary neurotrophic factor on neural differentiation of stem cells of human exfoliated deciduous teeth
- PDF / 4,621,139 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 89 Downloads / 185 Views
(2020) 14:29
RESEARCH
Open Access
Effect of ciliary neurotrophic factor on neural differentiation of stem cells of human exfoliated deciduous teeth Sujuan Zeng1, Xuedan Zhao1, Lingling Zhang1,2, Janak L. Pathak1, Wenyan Huang1, Yunyang Li1, Hongbing Guan1, Wanghong Zhao3*, Lihong Ge1* and Yan Shu1,4*
Abstract The stem cells of human exfoliated deciduous teeth (SHEDs) are considered to be one of the main sources of seed cells in stem cell therapy. The aim of this study was to examine the effect of ciliary neurotrophic factor (CNTF) on neurogenic differentiation of SHEDs. With the consent of parents, SHEDs from 6 to 8 year old children were isolated and cultured. The mesenchymal stemness and the potential of multidirectional (adipogenic and osteogenic) differentiation for the isolated SHEDs were firstly determined. The effect of CNTF on specific neurogenic differentiation of SHEDs was then examined by detecting the expression of marker genes and proteins via RT-PCR, immunoblotting, and immunofluorescence microscopy. The isolated SHEDs expressed specific surface markers of mesenchymal stem cells, and their potential of osteogenic and adipogenic differentiation were confirmed. CNTF promoted the differentiation of SHEDs into neuron-like cells with a high expression of acetylcholine transferase (CHAT), a marker of cholinergic neurons. The expression of other neuron markers including nestin, microtubuleassociated protein 2 (MAP 2), and β-tublin III was also detected. Interestingly, the expression of neurogenic markers was maintained at a high level after neurogenic induction. SHEDs can be induced by CNTF to differentiate into cholinergic neuron-like cells under appropriate culture conditions. Our findings have laid a foundation for future use of SHEDs to treat neurological diseases. Keywords: Ciliary neurotrophic factor, Stem cells of human exfoliated deciduous teeth, Neurogenic differentiation, Cholinergic neuron
Introduction Stem cells are those with the potential to differentiate into different types of cells in the body. Growth factors can regulate the differentiation of stem cells. With appropriate growth factors and culture conditions, stem cells have been widely explored as seed cells in cell * Correspondence: [email protected]; [email protected]; [email protected] 3 Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China 1 Department of Pediatric Dentistry, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou 510182, China Full list of author information is available at the end of the article
therapy which is used to replace the cells that are dysfunctional or dead, thereby restoring certain body function [1]. For example, bone marrow transplantation is the most widely used stem cell therapy, and cord bloodderived stem cells are also used in some treatments [2]. Choosing suitable stem cells and inducing them to become target cells, tissues, and organs
Data Loading...
