Synthesis of Molecularly Imprinted Polymer and Its Application as Solid-Phase Extraction Sorbent for Ceftazidime Determi
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Synthesis of Molecularly Imprinted Polymer and Its Application as Solid-Phase Extraction Sorbent for Ceftazidime Determination in Human Serum and Urine Samples Parisa Jaoshania and Ameneh Porgham Daryasaria, * a
Department of Chemistry, Lahijan Branch, Islamic Azad University, Lahijan, Iran *e-mail: [email protected] Received January 31, 2018; revised August 22, 2018; accepted March 12, 2020
Abstract—A novel molecularly imprinted solid–phase extraction method was developed coupled with HPLC for ceftazidime determination in biological fluids. Various parameters such as molecular recognition properties, adsorption capacity and selectivity of the molecularly imprinted polymer (MIP) were evaluated. The optimal conditions for solid-phase extraction consisted of conditioning with 5 mL of methanol and 20 mL of deionized water at neutral pH, aqueous sample loading (50 mL at pH 3.0), washing using 5 mL of acetone and elution with 5 mL of acetonitrile–water solution (2 : 8, v/v). The MIP selectivity was evaluated by checking several substances with molecular structures similar to that of ceftazidime. The MIP relative standard deviation was 4.1% (n = 5). The limits of detection and quantification for ceftazidime were 7 and 25 μg/L, respectively, while linearity range was 25–800 μg/L. Keywords: ceftazidime, molecularly imprinted polymer, solid–phase extraction DOI: 10.1134/S1061934820090129
Ceftazidime is a third-generation cephalosporin with a widespread antibacterial activity against microorganisms particularly Pseudomonas aeruginosa and used for the therapy of infections in patients [1, 2] including respiratory, abdominal, meningitis and cystic fibrosis [3]. So far, several analytical methods such as chromatographic [4–6], spectrophotometric [7, 8] and electrochemical [9] have been applied for the determination of ceftazidime in biological fluids. However, the sensitivity requires to be progressed. Therefore, it is necessary to improve a simple and selective method for the determination of ceftazidime in real samples. Sample preparation is very important for the analysis of biological samples. Solid-phase extraction (SPE) was applied as a clean-up technique due to such advantages as simplicity, cost and consumption of small amounts of organic solvent [10]. In SPE with conventional sorbents, many interfering compounds are co-extracted due to non-specific interaction, so further clean-up is needed. However, owing to the insufficient selectivity of commercial SPE sorbents, molecularly imprinted polymers (MIPs) have been developed as enrichment sorbents to recognize target molecule from other similar compounds [11–13]. Molecularly imprinted polymers are synthesized by polymerization of functional monomer and cross-link in the presence of template analyte through covalent
or non-covalent interactions. When the templates are removed, binding sites within the polymer matrix are adapted to three-dimensional shape and functionalities of template molecules [14–16]. Molecularly imprinted polymers possess several advantages
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