Synthesis of single-cell oil by Yarrowia lipolytica MTCC 9520 utilizing slaughterhouse lipid waste for biodiesel product
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ORIGINAL ARTICLE
Synthesis of single-cell oil by Yarrowia lipolytica MTCC 9520 utilizing slaughterhouse lipid waste for biodiesel production P. Radha 1 & Sanjana Narayanan 1 & Angana Chaudhuri 1 & Sameena Anjum 1 & Deborah Lilly Thomas 1 & Ritwik Pandey 1 & K. Ramani 1 Received: 11 July 2020 / Revised: 19 October 2020 / Accepted: 6 November 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The present study explores biodiesel production from the single-cell oil (SCO) synthesized by Yarrowia lipolytica MTCC 9520, utilizing slaughterhouse lipid waste, goat tallow, as the carbon substrate. Various parameters, including cultivation time (96 h), pH(6), substrate concentration (1.5%, v/v), inoculum size (5%, v/v), and C/N ratio (100), were optimized to attain the maximum biomass and lipid yield and lipid content of 3.8 g/L, 2.6 g/L, and 69.3% (g/g dry weight), respectively. The presence of intracellular lipid bodies in Y. lipolytica was confirmed by observing the Nile red-stained cells by fluorescence microscopy. Besides, the fluorescence intensities of the intracellular lipid bodies were determined by the flow cytometer. The extracted SCO from Y. lipolytica MTCC 9520 was analyzed by Fourier transform infrared (FT-IR) spectroscopy. Gas chromatography-mass spectrometry (GC-MS) analysis of the transesterified SCO presented the fatty acid methyl ester (FAME) profile, including palmitic acid (42.9%), stearic acid (21.5%), myristic acid (18.3%), and oleic acid (7.0%). The obtained FAME composition was further used to predict the properties of the biodiesel. The revealed characteristics of the transesterified FAMEs signify the candidature of the synthesized SCO as an alternate feedstock for biodiesel production. Keywords Single-cell oil . Yarrowia lipolytica MTCC 9520 . Goat tallow . Fatty acid methyl esters . Biodiesel
1 Introduction In recent years, the use of fossil fuels has raised serious environmental concerns. Furthermore, petroleum-based resource availability has plummeted remarkably with an upsurge in worldwide exigency for energy [1]. Biofuels are customarily advocated as a cost-effective and environmentally benign alternative to petroleum and other fossil fuels. Biodegradability and descended toxicity of biodiesel ascertain it as a superior substitute for petroleum-based fuels [2]. Biodiesel can be utilized in ternary fuel blends, meaning a mixture of diesel, biodiesel, and bioethanol, in diesel engines [3]. The primary feedstock derived from plant sources like rapeseed oil, soybean oil, palm oil, etc. is used for commercial biodiesel production.
* K. Ramani [email protected] 1
Biomolecules and Biocatalysis Laboratory, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kancheepuram District, Kattankulathur, Tamil Nadu 603203, India
More than 95% of feedstock comes from edible oils since they are produced in many regions. The properties of biodiesel produced from these oils are appropriate as a substitute for diesel [4]. However, agricult
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