Yarrowia lipolytica as a Cell Factory for Oleochemical Biotechnology
The yeast Y. lipolytica has developed a very efficient mechanism in the degradation and utilization of hydrophobic substrates. In addition, its capacity to accumulate great amounts of lipids places it among the oleaginous yeasts. The completion of the seq
- PDF / 100,956 Bytes
- 8 Pages / 439.37 x 666.142 pts Page_size
- 67 Downloads / 187 Views
1
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3004
2 2.1 2.2 2.3 2.4
Applications of Y. lipolytica in Oleochemical Biotechnology . . . . . . . . . . . . . . . . . . . . . 3004 Single Cell Oils . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3004 PUFA Production . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3006 Fine Chemistry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3006 Bioremediation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3008
3
Research Needs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3008
K. N. Timmis (ed.), Handbook of Hydrocarbon and Lipid Microbiology, DOI 10.1007/978-3-540-77587-4_223, # Springer-Verlag Berlin Heidelberg, 2010
3004
45
Yarrowia lipolytica as a Cell Factory for Oleochemical Biotechnology
Abstract: The yeast Y. lipolytica has developed a very efficient mechanism in the degradation and utilization of hydrophobic substrates. In addition, its capacity to accumulate great amounts of lipids places it among the oleaginous yeasts. The completion of the sequence of the Y. lipolytica genome and the existence of genetic tools for gene manipulation allow the use of its metabolic functions for biotechnological applications. Examples are presented demonstrating that wild type and genetically engineered strains of Y. lipolytica can be used for fatty acid bioconversion, substrate valorization, polyunsaturated fatty acids production, oil polluted water bioremediation and single cell oil production. This review also presents the potential uses of this yeast as an alternative production (cell) factory in the oleochemical field.
1
Introduction
The application of biotechnology to industrial production holds many promises for sustainable development, but many products still have to prove their economic viability. In the start of the twentieth century organic chemistry developed methods to create energy, clothing, synthetic polymers and plastics from oil. However, synthetic biology may be having its turn to play in the field. Stricter environmental regulations, the growing mass of non- degradable synthetics, and growing concerns about the dependence on imported oil, together with its price are in its favor. Exploring the nature’s diversity and using the already existing biological functions of organisms to produce molecules with equivalent or even improved properties compared with those derived from fossil will be a challenge. This review will focus on the potential applications of Y. lipolytica, an oleaginous yeast, in t
Data Loading...
