Procyanidin B2 Improves Oocyte Maturation and Subsequent Development in Type 1 Diabetic Mice by Promoting Mitochondrial
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REPRODUCTIVE BIOLOGY: ORIGINAL ARTICLE
Procyanidin B2 Improves Oocyte Maturation and Subsequent Development in Type 1 Diabetic Mice by Promoting Mitochondrial Function Yuxi Luo 1 & Qingrui Zhuan 1 & Jun Li 2 & Xingzhu Du 1 & Zhengyuan Huang 3 & Yunpeng Hou 4 & Xiangwei Fu 1 Received: 2 April 2020 / Revised: 6 June 2020 / Accepted: 15 June 2020 # Society for Reproductive Investigation 2020
Abstract Type 1 diabetes (T1D) results in decreased oocyte quality and compromised early embryonic development. Procyanidin B2 (PB2) is a natural compound extracted from grape seeds and has strong antioxidant activity in vivo. This study evaluated the effect of PB2 on oocyte maturation in diabetic mice. Diabetic mice were induced by streptozotocin (STZ) injection. PB2 was supplemented in the in vitro maturation medium, and the ratio of germinal vesicle breakdown (GVBD) and polar body extrusion (PBE), reactive oxygen species (ROS) levels, mitochondrial function, developmental ability, as well as crotonylation at H4K5 were determined in oocytes. PB2 can promote the extrusion of PBE (88.34% vs. 75.02%, P < 0.05); reduce the generation of ROS (1.12 vs. 1.96, P < 0.05); and improve the level of mitochondrial membrane potential (0.87 vs. 0.79 Δφm, P < 0.05), ATP level (1.31 vs. 0.71 pmol, P < 0.05), and mitochondria temperature (618.25 vs. 697.39 pixels, P < 0.05). The addition of PB2 also improved the level of oocyte crotonylation at H4K5 (crH4K5) (47.26 vs. 59.68 pixels, P < 0.05) and increased the blastocyst rate (61.51% vs. 36.07%, P < 0.05) after parthenogenetic activation. Our results are the first to reveal a role for PB2 in promoting the viability of oocytes by regulating the mitochondrial function. Moreover, we uncover that PB2 can improve the level of crH4K5, which provides a new strategy to combat the decline in oocyte quality of diabetic. Keywords Oocyte . Embryo . Procyanidin B2 . Mitochondrial . Crotonylation . Mice
Introduction With the changes in lifestyle and dietary habits, the prevalence of diabetes is rising rapidly. Type 1 diabetes (T1D) is caused by Yuxi Luo and Qingrui Zhuan contributed equally to this work. * Xiangwei Fu [email protected] 1
National Engineering Laboratory for Animal Breeding, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
2
Department of Reproductive Medicine, The First Hospital of Hebei Medical University, Shijiazhuang 050031, Hebei, China
3
Chelsea and Westminster Hospital, Department of Metabolism, Digestion and Reproduction, Imperial College London, London SW10 9NH, UK
4
State Key Laboratory of Agro biotechnology, College of Biological Sciences, China Agricultural University, Yuanmingyuan West Rd 2, Haidian District, Beijing 100193, China
autoimmune damage to the beta cells of the pancreas, resulting in a severely absolute lack of insulin that requires lifelong insulin treatment. According to epidemiological analysis, the prevalence of T1D is on the rise [1]. However, the path
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