Tannic acid-modified silver nanoparticles enhance the anti- Acanthamoeba activity of three multipurpose contact lens sol
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SHORT REPORT
Tannic acid‑modified silver nanoparticles enhance the anti‑Acanthamoeba activity of three multipurpose contact lens solutions without increasing their cytotoxicity Edyta B. Hendiger1,2* , Marcin Padzik1, Agnieszka Żochowska1, Wanda Baltaza1, Gabriela Olędzka1, Diana Zyskowska1, Julita Bluszcz1, Sylwia Jarzynka1, Lidia Chomicz1, Marta Grodzik3, Jacek Hendiger4, José E. Piñero2, Jarosław Grobelny5, Katarzyna Ranoszek‑Soliwoda5 and Jacob Lorenzo‑Morales2
Abstract Background: Free-living amoebae of the genus Acanthamoeba are cosmopolitan, widely distributed protozoans that cause a severe, vision-threatening corneal infection known as Acanthamoeba keratitis (AK). The majority of the increasing number of AK cases are associated with contact lens use. Appropriate eye hygiene and effective contact lens disinfection are crucial in the prevention of AK because of the lack of effective therapies against it. Currently avail‑ able multipurpose contact lens disinfection systems are not fully effective against Acanthamoeba trophozoites and cysts. There is an urgent need to increase the disinfecting activity of these systems to prevent AK infections. Synthe‑ sized nanoparticles (NPs) have been recently studied and proposed as a new generation of anti-microbial agents. It is also known that some plant metabolites, including tannins, have anti-parasitic activity. The aim of this study was to evaluate the anti-amoebic activity and cytotoxicity of tannic acid-modified silver NPs (AgTANPs) conjugated with selected multipurpose contact lens solutions. Methods: The anti-amoebic activities of pure contact lens care solutions, and NPs conjugated with contact lens care solutions, were examined in vitro by a colorimetric assay based on the oxido-reduction of alamarBlue. The cytotoxic‑ ity assays were performed using a fibroblast HS-5 (ATCC CRL-11882) cell line. The results were statistically analysed by ANOVA and Student-Newman-Keuls test using P < 0.05 as the level of statistical significance. Results: We show that the NPs enhance the anti-Acanthamoeba activities of the tested contact lens solutions without increasing their cytotoxicity profiles. The activities are enhanced within the minimal disinfection time recom‑ mended by the manufacturers. Conclusions: The conjugation of the selected contact lens solutions with AgTANPs might be a novel and promising approach for the prevention of AK infections among contact lens users. Keywords: Acanthamoeba keratitis, Contact lens solutions, Silver nanoparticles, Tannic acid
*Correspondence: [email protected] 1 Laboratory of Parasitology, Department of Medical Biology, Medical University of Warsaw, 14/16 Litewska Street, 00‑575 Warsaw, Poland Full list of author information is available at the end of the article © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appro
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