Silver Nanoparticles Stabilized with Chitosan Succinamide: Synthesis and Antibacterial Activity
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er Nanoparticles Stabilized with Chitosan Succinamide: Synthesis and Antibacterial Activity V. A. Alexandrovaa, *, A. M. Futoryanskayaa, **, and V. S. Sadykovab a
Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Moscow, 119991 Russia b Gause Institute of New Antibiotics, Moscow, 119021 Russia *e-mail: [email protected] **e-mail: [email protected] Received March 12, 2020; revised April 6, 2020; accepted April 22, 2020
Abstract—A water-soluble chitosan derivative, chitosan succinamide, was used as a polymer matrix for the formation of silver nanoparticles via ion reduction under microwave irradiation in the presence of a D-glucose reducing agent. UV spectroscopy confirmed the presence of silver nanoparticles in the synthesized colloidal solution. Transmission electron microscopy showed that the synthesized silver nanoparticles had a spherical shape. The obtained diffractograms confirm the presence of ref lexes characteristic of the crystal lattice of metallic silver. In vitro studies showed that the synthesized colloidal solution of silver nanoparticles exhibits pronounced antibacterial activity against strains of the gram-positive bacteria Bacillus subtilis ATCC 6633 and Bacillus coagulans 429. Keywords: microwave synthesis, silver nanoparticles, chitosan succinamide, antibacterial activity DOI: 10.1134/S0003683820050026
INTRODUCTION The development of new nanocomposites is a rapidly developing area in science and technology at present. Thus, the creation of nanocomposite medical materials based on nontoxic biodegradable polymers of natural origin is an urgent task. Polysaccharides, in particular, chitosan and its derivatives, can be used as such polymers. These materials can be used in the development of wound-healing medicines, implants for bone-tissue regeneration, tissue-engineering matrices, and controlled drug-delivery systems. From this point of view, metal-polymer nanocomposites are of great interest, since the inclusion of various metals in their composition, in particular, silver, makes it possible to obtain materials with desirable properties, including antimicrobial activity [1]. Chitosan is insoluble in water, and the preparation of its water-soluble derivatives, which are synthesized via introducing various functional groups into a macromolecule, can significantly expand the prospects of the use such a polymer in biology and medicine. Such derivatives include chitosan succinamide [2]. The biological activity of succinamide chitosan and the possibility of its use for the immobilization of medicinal substances are quite widely studied at present. It is important to note that N-succinyl chitosan is safe for intravenous administration [3, 4], which allows
the use of the polymer in delivery systems of anticancer drugs [5]. In addition, N-succinyl chitosan exhibits antiaggregative ntn and antioxidant activity [6]. Silver nanoparticles can be directly synthesized in the polymer solution. In this case, the polymer itself serves as a matrix that controls the size and shape
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