The ankyrin repeat-containing protein MdANK2B regulates salt tolerance and ABA sensitivity in Malus domestica
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ORIGINAL ARTICLE
The ankyrin repeat‑containing protein MdANK2B regulates salt tolerance and ABA sensitivity in Malus domestica Fu‑jun Zhang1 · Yin‑huan Xie1 · Han Jiang2 · Xun Wang1 · Yu‑Jin Hao1 · Zhenlu Zhang1 · Chun‑Xiang You1 Received: 23 July 2020 / Accepted: 23 November 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message The ankyrin repeat-containing protein MdANK2B was identified to contribute to increasing resistance to salt stress and decreasing sensitivity to ABA in Malus domestica. Abstract Ankyrin (ANK) repeat-containing proteins occur widely in prokaryotes, eukaryotes, and even in some viruses and play a critical role in plant growth and development, as well as the response to biotic and abiotic stress. However, the function of ANK repeat-containing proteins in apple (Malus domestica) has not yet been investigated. Here, we identified apple MdANK2B based on homology analysis with the Arabidopsis ANK repeat-containing proteins AtAKR2A and AtAKR2B. MdANK2B was found to be localized in the cytoplasm, and its encoding gene was highly expressed in both apple leaves and fruits. In addition, MdANK2B gene expression was highly induced by salt stresses and abscisic acid (ABA). Overexpression of MdANK2B increased resistance to salt stress and decreased sensitivity to ABA in both transgenic apple calli and seedlings. In addition, overexpression of MdANK2B reduced the accumulation of reactive oxygen species (ROS) by enhancing the activity of antioxidant enzymes in response to salt stress. Our data revealed the role of MdANK2B in response to salt stress and ABA treatment in apple, which widens the known functions of ANK repeat-containing proteins in response to abiotic stress. Keywords Apple (malus domestica) · Ankyrin repeat-containing protein · MdANK2B · Salt stress · ROS · ABA sensitivity Abbreviations ANK Ankyrin ROS Reactive oxygen species ABA Abscisic acid ITN1 Increased tolerance to NaCl1 RBOHC Respiratory Burst Oxidase Homolog C Communicated by Qiaochun Wang. Supplementary Information The online version of this article (https://doi.org/10.1007/s00299-020-02642-9) contains supplementary material, which is available to authorized users. * Zhenlu Zhang [email protected] * Chun‑Xiang You [email protected] 1
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National Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, National Research Center for Apple Engineering and Technology, Shandong Agricultural University, Tai‑An 271018, Shandong, China State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yang ling, Shanxi 712100, China
RBOHD Respiratory Burst Oxidase Homolog D COM Chloroplast outer membrane RPL23A Ribosomal Protein L23A KCS1 3-Ketoacyl-CoA Synthase1 VLCFA Very long-chain fatty acids MS Murashige and Skoog 2, 4-D 2, 4-Dichlorophenoxyacetic acid 6-BA 6-Benzylaminopurine NAA Naphthyl acetate EL Electrolyte leakage ORF Open reading frame CAT Catalase POD Peroxidase DAB Diaminobenzidine
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