MKK3-MPK6-MYC2 module positively regulates ABA biosynthesis and signalling in Arabidopsis
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ORIGINAL ARTICLE
MKK3-MPK6-MYC2 module positively regulates ABA biosynthesis and signalling in Arabidopsis Deepanjali Verma1
•
Prakash Kumar Bhagat1 • Alok Krishna Sinha1
Received: 15 July 2020 / Accepted: 6 October 2020 Ó Society for Plant Biochemistry and Biotechnology 2020
Abstract Involvement of MAPK cascades is well studied in the regulation of ABA mediated responses such as stomatal opening, seed germination and stress tolerance. However most of the studies have focused on ABA signalling and involvement of MAPKs in ABA biosynthesis remains elusive. Hence, in the current study, we have identified the new role of MKK3MPK6-MYC2 module in positive regulation of ABA biosynthesis as well as ABA responsive genes. Seed germination studies disclosed the ABA insensitive phenotype of myc2, mpk6 and mkk3 mutants. Reduced expression of ABA biosynthesis genes, ABA2 and NCED3 in myc2 and MAPK cascade mutants was confirmed by witnessing the direct binding of MYC2 to the promoter of ABA2 in EMSA assays. Additionally, ABA responsive genes also displayed altered expression patterns in these mutants. Further, transcript and biochemical studies validated the role of MPK6 upstream to MYC2 and downstream to MKK3 in response to ABA. Altogether, we have shown that MKK3-MPK6-MYC2 module induces the expression of several ABA related genes and regulate ABA mediated inhibition of seed germination. Keywords ABA MYC2 ABA2 MPK6 MKK3 Seed germination Abbreviations ABA ABA2 NCED3 MPK6 MKK3 MAP3K17/18 ANAC019 AHK5 GSTU17
Abscisic acid ABA deficient 2 Nine-cisepoxycarotenoid dioxygenase 3 MAP kinase 6 MAP kinase kinase 3 MAP kinase kinase kinase 17/18 NAC domain containing protein 19 Histidine Kinase 5 Glutathione S-transferase U17
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13562-020-00621-5) contains supplementary material, which is available to authorized users. & Alok Krishna Sinha [email protected] Deepanjali Verma [email protected] Prakash Kumar Bhagat [email protected] 1
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India
Introduction Abscisic acid (ABA) is master regulator of abiotic stress adaptation responses. It plays crucial roles in the regulation of several aspects of plant growth and development such as seed dormancy, germination, maturation, seedling growth, vegetative growth, flowering, senescence, stomatal movement, embryo development, and stress-tolerance responses. (Chiwocha et al. 2005; Finkelstein 2013). ABA is a type of metabolite, which is known as isoprenoids, or terpenoids and is classified as sesquiterpene (Nambara and MarionPoll 2005). ABA is synthesized from b-carotene by an array of enzymes such as zeaxanthin oxidase (ZEP), 9-cisepoxycarotenoid dioxygenase (NCED), ABA deficient 2 (ABA2), ABA-aldehyde oxidase (AAO) and molybdenum cofactor sulfurase (MCSU) (Tuteja 2007). ABA performs an array of functions at cell level such as controlling the production of enzymes required to protect cells from dehydratio
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