The ASHR3 SET-Domain Protein is a Pivotal Upstream Coordinator for Wound-Induced Callus Formation in Arabidopsis
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RESEARCH ARTICLE
The ASHR3 SET‑Domain Protein is a Pivotal Upstream Coordinator for Wound‑Induced Callus Formation in Arabidopsis Kyounghee Lee1 · Ok‑Sun Park1 · Hong Gil Lee1,2 · Pil Joon Seo1,2 Received: 16 March 2020 / Revised: 29 April 2020 / Accepted: 29 May 2020 © Korean Society of Plant Biologist 2020
Abstract Plants can induce callus formation at damaged tissue to replenish cellular damages and facilitate tissue regeneration. Physical wounding triggers a transcriptional signaling cascade to induce cell division for callus formation. Several transcriptional regulators involved in wound-induced callus formation have been identified, but how they are coordinated remains elusive. Here, we report that a SET-domain-containing protein, ASH1-RELATED 3 (ASHR3), acts as an upstream regulator of ARABIDOPSIS RESPONSE REGULATOR 1 (ARR1), PLETHORA 3 (PLT3), and WOUND-INDUCED DEDIFFERENTIATION 3 (WIND3) and promotes wound-induced callus formation. Expression of ASHR3 was rapidly induced by wounding, and the ASHR3 protein possibly catalyzed H3K36me3 deposition at the ARR1, PLT3, and WIND3 loci to activate gene expression. In support, loss-of-function mutants of ASHR3 displayed reduced callus formation after wounding on hormonefree medium. Collectively, our study indicates that wounding activates a chromatin modifier, which establishes chromatin landscapes optimizing cell proliferation and reprogramming. Keywords ASHR3 · Callus · Epigenetics · Wounding
Introduction Plants have a remarkable ability to induce pluripotent stem cell mass, callus, from differentiated plant tissues (Ikeuchi et al. 2013; Feher 2019). The reversible transition of cell identities provides biological advantages to overcome sessile nature of plants and ensures developmental plasticity in response to environmental fluctuations (Feher 2019). The plant regeneration process is particularly important for healing of damaged tissues. Wound-induced callus can derive from various cell types, including vascular cells, cortical cells, and pith cells (Ikeuchi et al. 2013), unlike hormoneinduced callus that derives from pericycle cells (Atta et al. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12374-020-09259-1) contains supplementary material, which is available to authorized users. * Pil Joon Seo [email protected] 1
Department of Chemistry, Seoul National University, Seoul 08826, South Korea
Plant Genomics and Breeding Institute, Seoul National University, Seoul 08826, South Korea
2
2009; Sugimoto et al. 2010). Then, wound-induced callus allows to replenish damaged tissues, based on cellular activities of proliferation and pluripotency (Ikeuchi et al. 2013). Several molecular factors involved in wound-induced callus formation have been identified. Cytokinin biosynthesis and signaling exert an important function in callus induction at wound sites. Cytokinin biosynthesis mutants, log123457 and ipt357 (Miyawaki et al. 2006; Tokunaga et al. 2012), and genetic mutants of type B-ARABIDOPSIS RESPONSE RE
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